Center for Caenorhabditis Elegans Anatomy

秀丽隐杆线虫解剖中心

• 批准号: 8609737
• 负责人: DAVID H. HALL
• 金额: $ 67.29万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-01 至 2018-03-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8609737
• 关键词: Adopted; Adult; Anatomy; Animal Model; Animals; Apoptosis; Archives; Atlases; Autophagocytosis; Books; Brain; C. elegans genome; Caenorhabditis elegans; Cell Death; Cellular biology; Collaborations; Commit; Communities; Data; Databases; Development; Disease; Drops; Electrons; Funding; Gene Mutation; Genes; Genetic; Genome; Goals; Histocytochemistry; Image; Knowledge; Laboratories; Light; Methods; Microscopic; Microtomy; Mission; Monoclonal Antibody R24; Necrosis; Nematoda; Pathology; Postdoctoral Fellow; Proteins; Publishing; Research; Research Project Grants; Staging; Structure; Students; Surveys; Techniques; Technology; Testing; Text; Training; United States National Institutes of Health; base; cellular development; man; method development; public health relevance; research and development; research study; success; web site

DESCRIPTION (provided by applicant): The nematode, Caenorhabditis elegans, has been identified as a "high connectivity" animal for cellular development. This very small, simple animal has been widely adopted for experimental study due to its excellent genetics and accessible cell biology. The complete genome of the worm has been published, and most of the adult anatomy has been reconstructed from electron micrographs of serial thin sections. However, EM techniques are not widely utilized by the C. elegans community, and most previous EM data are not easily accessed. The Center for C. elegans Anatomy concentrates on 1) publishing detailed information regarding the anatomy of the nematode in the form of annotated TEM and light micrographs, available on our two websites WormAtlas (text-based) and Wormlmage (image database), and by ftp, on hard drives, and in book form as the C. elegans Atlas. The Center is 2) providing practical training in modern EM methods for students and postdoc's and 3) testing new EM methods for C. elegans. We also conduct new EM surveys of the wild type anatomy at key developmental stages to supplement the Adas, and to fill gaps in our general knowledge. This R24 application was first funded and the Center opened in Feb. 1998; we request years of funding to continue building our capabilities to serve the scientific community.

描述（由申请人提供）：线虫秀丽隐杆线虫已被确定为细胞发育的“高连接性”动物。这种非常小、简单的动物由于其优异的遗传学和易于理解的细胞生物学而被广泛用于实验研究。该蠕虫的完整基因组已发表，并且大部分成虫解剖结构都是根据连续薄片的电子显微照片重建的。然而，电镜技术并未被线虫群体广泛使用，而且大多数以前的电镜数据也不容易获取。线虫解剖学中心专注于 1) 以带注释的 TEM 和光学显微照片的形式发布有关线虫解剖学的详细信息，这些信息可以在我们的两个网站 WormAtlas（基于文本）和 Wormmage（图像数据库）上获取，也可以通过 ftp 在硬盘上获取，并以书籍形式作为线虫图集获取。该中心 2) 为学生和博士后提供现代 EM 方法的实践培训，3) 测试线虫的新 EM 方法。我们还在关键发育阶段对野生型解剖学进行了新的 EM 调查，以补充 Adas，并填补我们常识的空白。该 R24 申请首次获得资助，该中心于 1998 年 2 月开业；我们请求多年的资金来继续建设我们为科学界服务的能力。