IN VIVO ACUTE JOINT TRAUMA

体内急性关节创伤

• 批准号: 2081164
• 负责人: ROBY C THOMPSON
• 金额: $ 21.48万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-01-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2081164
• 关键词: articular cartilage; biomechanics; chondrocytes; collagen; disease /disorder model; dogs; enzyme mechanism; fluorescence microscopy; in situ hybridization; joint disorder; laboratory rabbit; osteoarthritis; proteoglycan; technology /technique development; tissue /cell culture; trauma; wound healing

The long term objectives are to understand the degradation and repair processes that occur after acute joint trauma. A canine model system of acute transarticular load will be used to generate tissue for an In vitro investigation on the role that matrix damage plays chondrocyte mediated matrix degradation in the early phase of degeneration. The mechanisms, whereby matrix damage plays a role in the response, will be investigated using a combination of quantitative biochemistry, histology, immunohistochemistry, and in situ hybridization. A biomechanical model of the initial damage will be developed. The in vivo response to the acute damage to the weight bearing area of the medial femoral condyle will be followed. After the temporal sequence is established, the model will be perturbed by anterior cruciate ligament transection. The role of altered forces and motions on acutely damaged cartilage will then be determined. Information from this study will lead to a better understanding the role acute joint trauma plays in the subsequent development of osteoarthritis. Detailed knowledge of the early events in the degradative phase that often occur after the initial trauma, could lead to the rational planning of Intervention for post-traumatic osteoarthritis as well as a better understanding of the osteoarthritic process.

长期目标是了解退化和修复 急性关节创伤后发生的过程。犬模型系统 急性经关节载荷将用于生成组织，以进行体外 软骨细胞介导基质损伤的研究 在退化的早期阶段基质降解。机制， 由此矩阵损伤在响应中起作用，将进行研究 使用定量生物化学，组织学， 免疫组织化学和原位杂交。 的生物力学模型 初始损伤将发展。体内对急性 股骨内侧髁的承重区域的损伤将 跟踪了时间序列建立后，模型将 前交叉韧带断裂的困扰改变的作用 然后将确定急性损伤软骨上的力和运动。 本研究的信息将有助于更好地了解 急性关节创伤在骨关节炎的后续发展中起作用。 对降解阶段早期事件的详细了解， 发生在最初的创伤后，可能会导致合理的计划， 创伤后骨关节炎的干预以及更好的 了解骨关节炎的过程。