IN VIVO ACUTE JOINT TRAUMA

长期目标是了解退化和修复 在急性关节创伤后发生的过程。犬模型系统的建立 急性跨关节负荷将用于生成体外培养的组织 基质损伤在软骨细胞介导中的作用研究 基质降解的早期阶段。这些机制， 其中基质损伤在反应中扮演的角色，将被调查 利用定量生物化学、组织学、 免疫组织化学和原位杂交。一种生物力学模型的建立 最初的损害将被开发出来。急性呼吸窘迫综合征的体内反应 股骨内侧髁负重区的损伤将是 紧随其后。在时间序列建立之后，模型将被 被前十字韧带切断所困扰。被更改的角色 然后将确定急性损伤软骨上的力和运动。 从这项研究中获得的信息将有助于更好地理解 急性关节创伤在骨性关节炎的后续发展中起作用。 关于退化阶段的早期事件的详细知识，通常 发生在最初的创伤之后，可能会导致合理的规划 对创伤后骨关节炎的干预以及更好的 了解骨关节炎的发病过程。

The long term objectives are to understand the degradation and repair processes that occur after acute joint trauma. A canine model system of acute transarticular load will be used to generate tissue for an In vitro investigation on the role that matrix damage plays chondrocyte mediated matrix degradation in the early phase of degeneration. The mechanisms, whereby matrix damage plays a role in the response, will be investigated using a combination of quantitative biochemistry, histology, immunohistochemistry, and in situ hybridization. A biomechanical model of the initial damage will be developed. The in vivo response to the acute damage to the weight bearing area of the medial femoral condyle will be followed. After the temporal sequence is established, the model will be perturbed by anterior cruciate ligament transection. The role of altered forces and motions on acutely damaged cartilage will then be determined. Information from this study will lead to a better understanding the role acute joint trauma plays in the subsequent development of osteoarthritis. Detailed knowledge of the early events in the degradative phase that often occur after the initial trauma, could lead to the rational planning of Intervention for post-traumatic osteoarthritis as well as a better understanding of the osteoarthritic process.