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IN VIVO ACUTE JOINT TRAUMA

体内急性关节创伤

基本信息

批准号：
2633651
负责人：
ROBY C THOMPSON
金额：
$ 24.63万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-01-01 至 1999-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2633651
关键词：
articular cartilage biomechanics chondrocytes collagen disease /disorder model dogs enzyme mechanism fluorescence microscopy in situ hybridization joint disorder laboratory rabbit osteoarthritis proteoglycan technology /technique development tissue /cell culture trauma wound healing

项目摘要

The long term objectives are to understand the degradation and repair processes that occur after acute joint trauma. A canine model system of acute transarticular load will be used to generate tissue for an In vitro investigation on the role that matrix damage plays chondrocyte mediated matrix degradation in the early phase of degeneration. The mechanisms, whereby matrix damage plays a role in the response, will be investigated using a combination of quantitative biochemistry, histology, immunohistochemistry, and in situ hybridization. A biomechanical model of the initial damage will be developed. The in vivo response to the acute damage to the weight bearing area of the medial femoral condyle will be followed. After the temporal sequence is established, the model will be perturbed by anterior cruciate ligament transection. The role of altered forces and motions on acutely damaged cartilage will then be determined. Information from this study will lead to a better understanding the role acute joint trauma plays in the subsequent development of osteoarthritis. Detailed knowledge of the early events in the degradative phase that often occur after the initial trauma, could lead to the rational planning of Intervention for post-traumatic osteoarthritis as well as a better understanding of the osteoarthritic process.

长期目标是了解退化和修复急性关节创伤后发生的过程。犬类模型系统急性经关节负荷将用于生成用于体外的组织基质损伤在软骨细胞介导中的作用研究退化早期的基质退化。机制，将研究基质损伤在响应中发挥的作用结合定量生物化学、组织学、免疫组织化学和原位杂交。生物力学模型最初的损害将会发展。体内对急性的反应股骨内侧髁的承重区域会受到损害紧随其后。时间序列建立后，模型将因前交叉韧带横断而受到干扰。改变的角色然后将确定严重受损软骨上的力和运动。这项研究的信息将有助于更好地理解该角色急性关节创伤在骨关节炎的后续发展中发挥作用。对退化阶段早期事件的详细了解，通常发生在最初的创伤之后，可能会导致合理的计划创伤后骨关节炎的干预以及更好的治疗了解骨关节炎的过程。