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ANTIGENIC SPECIFICITY AND V REGION USE OF AIDS B NHLS

ANTIGENIC SPECIFICITY AND V REGION USE OF AIDS B NHLS
AIDS B NHLS 的抗原特异性和 V 区使用
批准号:
2096126
负责人:
MICHAEL Shannon MCGRATH
金额:
$22.6万
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-04-01 至 1995-03-31

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中文摘要
翻译
这项提议的总体目标是测试是否与艾滋病相关的b细胞
英文摘要
The overall goal of this proposal is to test whether AIDS-associated B-cell non-Hodgkin's lymphomas (NHL) represent outgrowths of viral or self antigen activated B-cells, to define the spectrum of immunoglobulin variable (V) region genes utilized by these lymphomas and to test whether subsets of lymphoma would utilize identical V regions. If viral antigens are found to drive the pre-malignant B-cell proliferation or common V region determinants are shared between different lymphomas, non-immunosuppressive therapeutic approaches to the prevention and treatment of AIDS-lymphomas could be devised. In a preliminary analysis of two AIDS lymphoma cell lines we found one AIDS NHL IgM to be HIV-1 reactive (2F7) and the other to be self reactive (10C9). Rather than laboriously and inefficiently establish other AIDS NHL cell lines, we will create AIDS NHL hybridomas for testing of IgM antigenic specificity. In order to rapidly clone V regions and compare V region sequences between large numbers of patient specimens, a recent advance in polymerase chain reaction (PCR) technology will allow the rapid and direct cloning of rearranged V regions from tumor genomic DNA to test whether, as in other classes of lymphoid malignancy, subsets of AIDS NHL will be found to utilize common V regions. Specific aims: 1) To test IgM's from monoclonal and polyclonal AIDS-associated lymphomas for reactivity with a panel of viral (HIV-1, EBV, CMV) and self antigens, and utilizing polymerase chain reaction (PCR), to clone the IgM variable region genes from at least ten monoclonal tumors in order to define the spectrum of V region genes (and V region modifications) used by these lymphomas. 2) To characterize the molecular nature of the monoclonal idiotype shared between the 10C9 self reactive lymphoma cell line and 5 of 21 monoclonal AIDS-associated lymphomas. 3) To use specific AIDS lymphoma V region (CDR2) oligonucleotides from the ten cloned VH regions including the 10C9 V region for analysis of PCR amplified VH genes from frozen and formalin fixed archival AIDS NHL specimens (currently greater than 125 at SFGH) to determine the prevalence of specific VH region utilization in AIDS-associated lymphomas.
期刊论文(7)
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会议论文
A non-lymphoma idiotype is indicative and predictive for B cell malignancies in AIDS.
非淋巴瘤独特型可指示和预测 AIDS 中的 B 细胞恶性肿瘤。
DOI: 10.1089/hyb.1993.12.529
发表时间: 1993
期刊: Hybridoma
影响因子: --
作者: [Herndier,B, McGrath,M, Abbey,N, Wang,HT, Ng,V, Dorigo,O, Kaplan,L, Muller,S, Kohler,H]
通讯作者: Kohler,H
IgMs produced by two acquired immune deficiency syndrome lymphoma cell lines: Ig binding specificity and VH-gene putative somatic mutation analysis.
两种获得性免疫缺陷综合征淋巴瘤细胞系产生的 IgM:Ig 结合特异性和 VH 基因推定体细胞突变分析。
DOI: --
发表时间: 1994
期刊: Blood
影响因子: 20.3
作者: [Ng,VL, Hurt,MH, Fein,CL, Khayam-Bashi,F, Marsh,J, Nunes,WM, McPhaul,LW, Feigal,E, Nelson,P, Herndier,BG]
通讯作者: Herndier,BG
AIDS associated non-Hodgkin's lymphomas represent a broad spectrum of monoclonal and polyclonal lymphoproliferative processes.
艾滋病相关的非霍奇金淋巴瘤代表了广泛的单克隆和多克隆淋巴增殖过程。
DOI: 10.1007/978-3-642-77633-5_49
发表时间: 1992
期刊: Current topics in microbiology and immunology
影响因子: --
作者: [Herndier,BG, Shiramizu,BT, McGrath,MS]
通讯作者: McGrath,MS
VH gene use by HIV type 1-associated lymphoproliferations.
HIV 1 型相关淋巴细胞增殖使用 VH 基因。
DOI: 10.1089/aid.1997.13.135
发表时间: 1997
期刊: AIDS research and human retroviruses.
影响因子: --
作者: [Ng,VL, Hurt,MH, Herndier,BG, Fry,KE, McGrath,MS]
通讯作者: McGrath,MS
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