NOVEL HOLLOW-FIBER BIOREACTOR WITH INTEGRAL OXYGENATION

描述：(改编自申请者摘要)本报告的目的 二期工程是进一步开发一种新设计的中空纤维 生物反应器。申请者声称该生物反应器可以支持五次 比现有的中空纤维生物反应器更具活力的哺乳动物细胞/毫升。 第一阶段的项目表明，由一个 内氧合膜可支持哺乳动物细胞的培养。 第二阶段项目将完成生物反应器的设计和 生成设计和开发一系列哺乳动物所需的数据 基于这一概念的细胞培养系统。第三阶段计划是 将一系列仪器商业化，从小型台式规模到 大规模的生产系统，这将满足 诊断和治疗蛋白质制造商。 第二阶段项目有两个主要研究领域：设计和 新反应堆的螺旋包装版本的开发 与依附细胞生长有关的能力。 对于前一项工作，使用了许多核磁共振成像和光谱技术 将被开发出来，使生物反应器的性能 非侵入性评估。申请人声明核磁共振技术可以 广泛适用于其他中空纤维生物反应器和高级细胞 密度灌流反应堆。

DESCRIPTION: (Adapted from Applicant's Abstract) The objective of this Phase II project is to further develop a newly designed hollow fiber bioreactor. The applicant claims the bioreactor can support five times more viable mammalian cells/ml than existing hollow fiber bioreactors. The phase I project demonstrated that a bioreactor constructed with an internal oxygenation membrane can support mammalian cells in culture. The Phase II project would complete the design of the bioreactor and generate the data required to design and develop a series of mammalian cell culture systems based on this concept. The Phase III plan is to commercialize a series of instruments, from small bench-top scale to large scale production systems, that would meet the needs of the diagnostic and therapeutic protein manufacturers. The Phase II project has two major areas of research: The design and development of a spirally-wrapped version of the new reactor's capabilities with respect to the growth of attachment dependent cells. For the former work a number of NMR imaging and spectroscopic techniques would be developed that would allow bioreactor performance to be evaluated non-invasively. The applicants states the NMR techniques may be widely applicable to other hollow fiber bioreactors and high cell density perfusion reactors.