NOVEL HOLLOW-FIBER BIOREACTOR WITH INTEGRAL OXYGENATION

具有整体氧化功能的新型中空纤维生物反应器

• 批准号: 3507034
• 负责人: Bruce Amiot
• 金额: $ 12.5万
• 依托单位: BIOVEST INTERNATIONAL, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-30 至 1995-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3507034
• 关键词: biomedical equipment development; bioreactors; confocal scanning microscopy; mass tissue /cell culture; nuclear magnetic resonance spectroscopy; oxygen

DESCRIPTION: (Adapted from Applicant's Abstract) The objective of this Phase II project is to further develop a newly designed hollow fiber bioreactor. The applicant claims the bioreactor can support five times more viable mammalian cells/ml than existing hollow fiber bioreactors. The phase I project demonstrated that a bioreactor constructed with an internal oxygenation membrane can support mammalian cells in culture. The Phase II project would complete the design of the bioreactor and generate the data required to design and develop a series of mammalian cell culture systems based on this concept. The Phase III plan is to commercialize a series of instruments, from small bench-top scale to large scale production systems, that would meet the needs of the diagnostic and therapeutic protein manufacturers. The Phase II project has two major areas of research: The design and development of a spirally-wrapped version of the new reactor's capabilities with respect to the growth of attachment dependent cells. For the former work a number of NMR imaging and spectroscopic techniques would be developed that would allow bioreactor performance to be evaluated non-invasively. The applicants states the NMR techniques may be widely applicable to other hollow fiber bioreactors and high cell density perfusion reactors.

描述：（改编自申请人的摘要）此目的 二期项目是进一步开发新设计的中空纤维 生物反应器。 申请人声称该生物反应器可支持五次 比现有的中空纤维生物反应器每毫升有更多的存活哺乳动物细胞。 第一阶段项目证明了用 内部氧合膜可以支持培养中的哺乳动物细胞。 二期项目将完成生物反应器的设计 生成设计和开发一系列哺乳动物所需的数据 基于此概念的细胞培养系统。 第三期计划是 将一系列仪器商业化，从小型台式规模到 大规模的生产系统，可以满足 诊断和治疗蛋白质制造商。 二期项目有两个主要研究领域：设计和 开发新反应堆的螺旋包裹版本 附着依赖性细胞生长的能力。 对于之前的工作，一些核磁共振成像和光谱技术 将开发出生物反应器的性能 非侵入性评估。 申请人指出 NMR 技术可以 广泛适用于其他中空纤维生物反应器和高细胞 密度灌注反应器。