FIBEROPTIC REFLECTANCE/PHOSPHORESCENCE SPECTROMETER

这项研究的长期目标是开发一种基于光纤的 反射仪器，用于非侵入性测量循环中的氧气， 细胞内组织区室。 第一阶段成功地证明了 采用曲线拟合光谱分析法 同时测量心外膜溶解氧和氧合和 心外膜组织中脱氧形式的肌红蛋白和血红蛋白。 这一方法将在第二阶段应用于 一种灵敏度提高的仪器，可以分辨血红蛋白、肌红蛋白 和线粒体细胞色素。 该装置的商业实用性将 通过应用于工作哺乳动物的研究， 心脏和骨骼肌缺血的研究。 的各项任务 在第二阶段，将： .确定曲线拟合反射率的最佳反射率标准 心外膜和骨骼肌光谱 .构建光纤耦合成像光谱仪， 同时在两个不同组织区域的组织氧 .同时评估组织氧监测仪的潜力 工作灌注时心外膜和心内膜氧合的监测 哺乳动物心脏 .进一步证明该工具的效用，通过其就业， 骨骼肌缺血的磁共振研究

The long term goal of this research is to develop a fiberoptic-based reflectance instrument to noninvasively measure oxygen in circulatory and intracellular tissue compartments. Phase I successfully demonstrated the feasibility of employing curve fitting spectral analysis to simultaneously measure epicardial dissolved oxygen and the oxygenated and deoxygenated forms of myoglobin and hemoglobin in epicardial tissue. This methodology will be applied during Phase II to the development of an instrument with increased sensitivity to resolve hemoglobin, myoglobin and mitochondrial cytochromes. The commercial utility of the device will be demonstrated through its application to studies of working mammalian hearts and studies of ischemia in skeletal muscle. The tasks to be undertaken during Phase II will be to: . determine optimal reflectance standards for curve fitting reflectance spectra from epicardium and skeletal muscle . construct an optical fiber-coupled imaging spectrograph to monitor tissue oxygen at two different tissue regions simultaneously . evaluate the Tissue Oxygen Monitor's potential by simultaneously monitoring oxygenation of epicardium and endocardium in working perfused mammalian heart . further demonstrate the instrument's utility through its employment in a magnetic resonance study of skeletal muscle ischemia