REGIONAL FLOW MEASUREMENT IN TUMORS

描述(逐字摘自申请者摘要)：这个项目的目标是 开发一种全自动化的高分辨率方法和仪器 肿瘤局部血流分布的三维标测。这 这项研究是基于一种工作假设：合适的荧光染料 可作为肿瘤血流的定量指标。存放 荧光微球，同时注射到动脉循环中 时间作为染料，用于校准血液的染料荧光测量 流。该项目将适应组织切片和视频仪器 影像对肿瘤局部血流的测定。第一阶段研究将 展示荧光染料提供高分辨率地图的能力 肿瘤的局部血流分布。 第一阶段研究成功，证明了高分辨率的可行性 绘制肿瘤内的血流图，将有助于制定 优化用于第二阶段研究的仪器和方法。第二阶段 将把这种流动方法的使用扩展到包括使用 多种颜色的荧光流动标记，包括荧光 肿瘤的检测和体积测量，以及描绘血管 肿瘤内形态计量学。改变肿瘤血流的治疗剂和 将对血管形成进行研究以证明血流成像的价值 肿瘤研究的方法。该项目将提供生理学和癌症 研究人员拥有一种独特的工具来量化区域流量和 肿瘤中的血管形成及其对血管生成影响的研究 这些参数上的抑制剂。 建议的商业应用： 这项技术将开发新的高分辨率成像方法来测量 肿瘤的局部血流。成像冷冻微型机瞄准的市场 作为这项研究的一部分，正在开发的方法是研究实验室 研究肿瘤生长和血管生成的组织。采用这些方法进行成像 肿瘤的局部血流有助于研究血管生成抑制剂的作用 对肿瘤生长和血流分布的影响。

DESCRIPTION (Verbatim From Applicant's Abstract): The goal of this project is to develop a fully automated method and instrument for high-resolution three-dimensional mapping of regional blood flow distribution in tumors. This research is based upon the working hypothesis that suitable fluorescence dyes may be used as quantitative indicators of blood flow in tumors. Deposition of fluorescent microspheres, injected into the arterial circulation at the same time as the dye, serve to calibrate the dye fluorescence measurement of blood flow. This project will adapt instrumentation for tissue sectioning and video imaging to determination of regional flow in tumors. Phase I research will demonstrate the ability of fluorescent dyes to provide high-resolution maps of regional blood flow distribution in tumors. Successful Phase I studies, demonstrating the feasibility of high-resolution mapping of blood flow in tumors, will aid in formulating design parameters for optimizing the instrument and methods for use in Phase II studies. Phase II will extend use of this flow methodology to studies that include the use of more than one color of fluorescent flow marker, that include fluorescent detection and volume measurement of tumors, and that delineate vascular morphometry within tumors. Therapeutic agents that modify tumor flow and vascularization will be studied to demonstrate the value of the flow imaging methods for tumor research. This project will provide physiology and cancer researchers with a unique tool for quantifying regional flow and vascularization in tumors and for studying the effects of angiogenesis inhibitors on these parameters. PROPOSED COMMERCIAL APPLICATION: This technology will develop new high-resolution imaging methods for measurement of regional blood flow in tumors. The market being targeted for the Imaging CryoMicrotome and the methods being developed as part of this research are research laboratories that study tumor growth and vascularization. Adoption of these approaches for imaging regional blood flow in tumors will benefit research on affects of angiogenesis inhibitors on tumor growth and blood flow distribution.