REGIONAL FLOW MEASUREMENT IN TUMORS

描述（逐字从申请人的摘要）：该项目的目标是 开发一种全自动的方法和仪器， 肿瘤局部血流分布的三维绘图。这 研究是基于工作假设， 可作为肿瘤血流的定量指标。沉积 荧光微球，同时注射到动脉循环中， 时间作为染料，用于校准血液的染料荧光测量 流该项目将调整组织切片和视频的仪器 成像以确定肿瘤中的局部血流。第一阶段研究将 展示了荧光染料提供高分辨率地图的能力， 肿瘤局部血流分布。 成功的第一阶段研究，证明了高分辨率 绘制肿瘤中的血流，将有助于制定设计参数， 优化用于II期研究的仪器和方法。二期 将把这种流动方法的使用扩展到包括使用 一种以上颜色的荧光流动标记物，包括荧光 肿瘤的检测和体积测量，以及描绘血管 肿瘤内的形态计量学。改变肿瘤流动的治疗剂， 将研究血管化以证明血流成像的价值 肿瘤研究的方法。这个项目将提供生理学和癌症 研究人员用一种独特的工具来量化区域流量， 肿瘤血管生成和研究血管生成的影响 这些参数的抑制剂。 拟定商业应用： 这项技术将开发新的高分辨率成像方法，用于测量 肿瘤的局部血流 成像冷冻切片机的目标市场 作为这项研究的一部分， 研究肿瘤生长和血管形成的机构。 采用这些方法进行成像 肿瘤局部血流有助于研究血管生成抑制剂的作用 对肿瘤生长和血流分布的影响

DESCRIPTION (Verbatim From Applicant's Abstract): The goal of this project is to develop a fully automated method and instrument for high-resolution three-dimensional mapping of regional blood flow distribution in tumors. This research is based upon the working hypothesis that suitable fluorescence dyes may be used as quantitative indicators of blood flow in tumors. Deposition of fluorescent microspheres, injected into the arterial circulation at the same time as the dye, serve to calibrate the dye fluorescence measurement of blood flow. This project will adapt instrumentation for tissue sectioning and video imaging to determination of regional flow in tumors. Phase I research will demonstrate the ability of fluorescent dyes to provide high-resolution maps of regional blood flow distribution in tumors. Successful Phase I studies, demonstrating the feasibility of high-resolution mapping of blood flow in tumors, will aid in formulating design parameters for optimizing the instrument and methods for use in Phase II studies. Phase II will extend use of this flow methodology to studies that include the use of more than one color of fluorescent flow marker, that include fluorescent detection and volume measurement of tumors, and that delineate vascular morphometry within tumors. Therapeutic agents that modify tumor flow and vascularization will be studied to demonstrate the value of the flow imaging methods for tumor research. This project will provide physiology and cancer researchers with a unique tool for quantifying regional flow and vascularization in tumors and for studying the effects of angiogenesis inhibitors on these parameters. PROPOSED COMMERCIAL APPLICATION: This technology will develop new high-resolution imaging methods for measurement of regional blood flow in tumors. The market being targeted for the Imaging CryoMicrotome and the methods being developed as part of this research are research laboratories that study tumor growth and vascularization. Adoption of these approaches for imaging regional blood flow in tumors will benefit research on affects of angiogenesis inhibitors on tumor growth and blood flow distribution.