IMAGE ANALYSIS OF VASCULAR CASTS

血管管型的图像分析

• 批准号: 6211157
• 负责人: Clyde H Barlow
• 金额: $ 42.77万
• 依托单位: BARLOW SCIENTIFIC, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6211157
• 关键词: artery; bioimaging /biomedical imaging; biomedical automation; biomedical equipment development; cardiovascular imaging /visualization; digital imaging; fluorescence; image processing; laboratory rat; measurement; microcirculation; sectioning

The goal of this research is to develop rapid, automated instrumentation procedures for detailed analysis of complete arterial networks in hearts. Measured parameters will include arterial diameter, frequency of arterial branching, branch lengths, and angles. This research is based on the working hypothesis that data for rapid reconstruction of detailed vascular maps can be obtained from digital fluorescence images of freshly exposed surfaces during serial sectioning of tissue blocks containing fluorescent vascular filling compound in the vascular network. During Phase I, an imaging cryomicrotome was modified to obtain vascula filling compound fluorescence images from rat hearts and lungs during serial sectioning. Optimal conditions for arterial filling and for image collection were explored. Prelminary analysis sofiware was developed. Phase II studies will involve correlations of vascular structure and vascular flow in heart and vascular structure and pulmonary function in lungs through collaborations with subcontractors at the University of Washington. During Phase II, efforts will be concentrated on soflware development to convert image data into databases sultable for physiological network analysis. This project will provide physiology and microcirculation researchers 'with an improved and affordable tool for vascular network mapping and for network analysis of vascular, pulmonary, neural and other networks in biological systems. PROPOSED COMMERCIAL APPLICATIONS: This technology would automate methods for evaluating vascular networks. Procedures that currently take months to years will be shortened to hours and days. Potential markets include research laboratories currently studying vascular branching and a large number of additional researchers who could now evaluate questions of vascular physiology that could not previously be answered due to time consumption and cost of data acquisition and analysis.

本研究的目标是开发快速，自动化的仪器程序，详细分析完整的动脉网络的心脏。测量参数包括动脉直径、动脉分支频率、分支长度和角度。这项研究是基于工作的假设，即数据的快速重建详细的血管地图，可以从数字荧光图像的新鲜暴露的表面在连续切片的组织块含有荧光血管填充化合物的血管网络。在第一阶段，一个成像冷冻切片机进行了修改，以获得血管填充化合物荧光图像从大鼠心脏和肺在连续切片。探讨了动脉充盈和图像采集的最佳条件。初步开发了分析软件。II期研究将通过与华盛顿大学分包商的合作，涉及心脏血管结构和血管流量以及肺部血管结构和肺功能的相关性。在第二阶段，工作将集中在软件开发，将图像数据转换成数据库，适用于生理网络分析。该项目将为生理学和微循环研究人员提供一个改进的和负担得起的工具，用于血管网络映射和生物系统中血管，肺，神经和其他网络的网络分析。拟议的商业应用：该技术将自动化评估血管网络的方法。目前需要数月至数年的程序将缩短为数小时和数天。潜在的市场包括目前正在研究血管分支的研究实验室和大量的其他研究人员，他们现在可以评估以前由于数据采集和分析的时间消耗和成本而无法回答的血管生理学问题。