INTRAMYOCARDIAL DISTRIBUTIONS OF MICROSPHERES FOR REGIONAL BLOOD FLOWS

评价多标记荧光微球的应用 用于测量大心脏局部心肌血流量 分辨率很高。评价应提供：(1)评估 相对于标准微球方法的准确性，(2)和 从人员、时间和方案编制方面对费用进行评估 处理数据和分析两者所需的开发时间 标准应用程序和要求更高的应用程序 检验正态分布中流量分布的分形性 心。由于使用拟议的荧光技术获得的数据 微球技术将提供准确的三维信息 在沉积密度方面，该项目允许同时 评价，收集有关的新信息 对冠状动脉解剖与临床关系的认识 分支网络和连续流的分布 生理状态，从加压素诱导的血管收缩到 运动和新陈代谢刺激引起的扩张状态。这个 NIH，NHLBI，研究基金，HL50238，题为“心脏代谢和 FLOWS“支持了这篇文章中提到的基础动物研究 建议；HL50238赠款的资金从1992年9月30日持续到 1997年8月31日。在这些方面没有重复的工作 相辅相成。具体目标：1.评估使用 一种记录单个微球位置的技术 心脏各室室心肌的三维结构 狗的心脏。2.确定治疗的有效性和准确性 从3维的分析中获得区域流动估计 数据，给定球面估计中的小误差的对偶问题 位置和相对较大的识别和批注错误 心脏内的区域体积，有界于不规则 心内膜和心外膜表面。3.确定可行性 ，以获取有关 使用已经开发的铸造技术在心脏中放置动脉床 这只填满了动脉系统，一直到末梢小动脉。

To evaluate the use of multiple-labelled fluorescent microspheres for the measurement of regional myocardial blood flows in large hearts with fine resolution. The evaluation should provide: (1) assessments of accuracy relative to the standard microsphere approach, (2) an evaluation of the costs in terms of personnel time and programming development time for handling the data and the analysis for both standard applications and the more demanding applications intended for examining the fractal nature of the flow distributions in the normal heart. Since the data obtained with the proposed fluorescent microsphere techniques will provide accurate 3-dimensional information on deposition densities, the project allows, simultaneously with the evaluation, the gathering of new information relevant to the understanding of the relationship between the anatomy of the coronary branching network and the distribution of flows in a succession of physiological states, from vasopressin-induced vasoconstriction to states of dilatation induced by exercise and metabolic stimulus. The NIH, NHLBI, research grant, HL50238, entitled "Cardiac Metabolism and Flows," supports the basic animal studies that are alluded to in this proposal; the HL50238 grant is funded from September 30, 1992 through August 31, 1997. There is no duplication of effort in these complementary efforts. Specific Aims: 1. To evaluate the use of a technique for recording the positions of individual microspheres in three dimensions within the myocardium of the cardiac ventricles of the hearts of dogs. 2. To determine the efficacy and accuracy of obtaining regional flow estimates from analysis of the 3-dimensional data, given the dual problems of minor errors in estimation of sphere position and relatively larger errors in identifying and annotating the regional volumes within the heart, bounded as it is with irregular endocardial and epicardial surfaces. 3. To determine the feasibility for obtaining information on the 3-dimensional arrangement of the arterial bed in the heart using an already developed casting technique that fills only the arterial system down to the terminal arterioles.