Measuring Oxygen and Hemoglobin Saturation Distributions in Skin Grafts

描述（由申请人提供）：本提案概述了一种新产品的开发，以及同时测量皮肤中皮下PO2和血红蛋白氧饱和度的新方法。该产品名为“Oxyspheres”，将广泛应用于皮肤移植、皮肤溃疡和截肢的治疗治疗。在皮肤和底层组织之间的界面测量PO2的能力，以及用单一产品测量皮肤中的血红蛋白饱和度，将有助于研究人员和临床医生评估氧气对愈合的影响，以及治疗对改变局部氧气可用性的影响。利用优化方法和多位点模型对不同氧浓度下的“氧球”发光光谱进行曲线拟合。通常，亚表面发光的多波长强度研究由于吸收成分（含氧和脱氧血红蛋白和黑色素）和组织光散射引起的波长依赖性光衰减而变得复杂。使用“Oxyspheres”的方法学方法将利用这些成分已知的光谱行为来拟合“Oxyspheres”发光发射光谱，并根据拟合参数确定PO2和血红蛋白氧饱和度。这种创新的方法代表了在测量伤口氧气可用性方面的重大进步，并且在使用荧光探针测量体内组织生化和生理方面也取得了重要进展。新方法将允许荧光发射的多波长分析，以量化上覆组织中分析物的浓度，同时补偿波长依赖的发射强度衰减。该方法的发展将遵循逐步发展，使用越来越复杂的样品基质，从缓冲液到血红蛋白溶液，再到皮肤移植物。第一阶段的研究为三个发展阶段中的每一个阶段都有明确的里程碑和可行性标准。第二阶段将推进产品和分析方法进入商业化产品，用于临床实践。

DESCRIPTION (provided by applicant): This proposal outlines the development of a new product, and a new methodological approach to the simultaneous measurement of subdermal PO2 and hemoglobin oxygen saturation in skin. The product, "Oxyspheres", will find wide use in the development of healing therapies for skin grafts, skin ulcers, and amputations. The capability to measure PO2 at the interface between skin and underlying tissues and to measure hemoglobin saturation in the skin with a single product will assist researchers and clinicians in evaluating the effects of oxygen on healing and the effects of therapies on altering local oxygen availability. The luminescence emission spectra of "Oxyspheres" at different oxygen concentrations will be curve fit using optimization methods and a multisite model. Typically, multiwavelength intensity studies of subsurface luminescence are complicated by wavelength-dependent attenuation of light by absorbing components (oxy- and deoxy-hemoglobin and melanin) and by tissue light scattering. The methodological approach for using "Oxyspheres" will take advantage of the known spectral behaviors of these components to fit "Oxyspheres" luminescence emission spectra and to determine PO2 and hemoglobin oxygen saturation from the fitting parameters. This innovative approach represents a significant advancement in measuring oxygen availability in wounds, and it also makes a critical advancement in using fluorescent probes to measure tissue biochemistry and physiology in vivo. The new methods will allow multiwavelength analysis of fluorescence emission to quantify concentrations of analytes in overlying tissues while compensating for wavelength dependent attenuation of emission intensities. Development of the method will follow a stepwise development using samples matrixes of increasing complexity, from buffer to hemoglobin solutions, to skin grafts. Phase I research has well defined milestones and feasibility criteria for each of the three stages of development. Phase II will advance the products and analytical methods into commercial products to be used in clinical practice. PUBLIC HEALTH RELEVANCE: This research will develop an innovative new product that can measure tissue oxygen tensions and hemoglobin oxygen saturations simultaneously. The ability to measure oxygen available at specific locations in tissue in addition to measuring oxygen available from the circulation provides a powerful new tool to physicians developing methods to enhance skin healing in patients suffering from diabetes, pressure ulcers, skin grafts, and amputations. The methodological approach applied to these problems promises to have general application to many fluorescent probes of cellular biochemistry and physiology and to become a generally applied method for analyzing tissue behavior in living systems.