STRUCTURE AND FUNCTION OF GP330

GP330的结构与功能

• 批准号: 2144809
• 负责人: WILLIAM SCOTT ARGRAVES
• 金额: $ 5.37万
• 依托单位: AMERICAN NATIONAL RED CROSS
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1996-01-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2144809
• 关键词: affinity chromatography; complementary DNA; genetic library; glycoprotein structure; human tissue; in situ hybridization; laboratory mouse; laboratory rabbit; laboratory rat; low density lipoprotein; molecular cloning; polymerase chain reaction; protein sequence; protein structure function; receptor binding; receptor expression; tissue /cell culture

The low density lipoprotein receptor (LDLR) is at the head of an emerging family of structurally and perhaps functionally related receptors. The family presently contains five members that include the LDLR, alpha2-macroglobulin receptor/low density lipoprotein receptor-related protein (alpha2MR/LRP), the 95 kDa avian oocyte-specific receptor for the yolk precursor vitellogenin, the avian 380 kDa ovarian follicle membrane protein, and the kidney proximal tubule membrane protein, glycoprotein 330 (gp330). Although gp330 has been implicated as the pathogenic antigen for Heymann nephritis, the rat model for membranous glomerulonephropathy, and it has been localized to coated-pits of kidney brush border epithelial cells, its function is not known. The emphasis of this proposal is on determining the structure and biological function of gp330. The objectives are to (a) determine the complete amino acid sequence of human gp330 by cDNA cloning, (b) to characterize the functional properties of gp330 by identifying its ligand(s) particularly in the context of renal epithelium where gp330 is highly expressed and, (c) to determine the expression pattern of gp330 in adult and embryonic tissues as well as in cultured cell lines. A related aim of this project is to isolate and characterize new members of the LDLR family using homology-PCR cloning and degenerate oligonucleotide screening of genomic libraries.

低密度脂蛋白受体(LDLR)是一种新兴的 结构上或功能上相关的受体家族。这个 该家族目前有五名成员，其中包括LDLR， α2-巨球蛋白受体/低密度脂蛋白受体相关 蛋白(alpha2MR/LRP)，95 kDa禽卵母细胞特异性受体 卵黄前体卵黄蛋白原，禽类380 kDa卵泡膜 蛋白，以及肾脏近端小管膜蛋白、糖蛋白 330(Gp330)。尽管gp330已被认为是致病因子 膜性Heymann肾炎大鼠模型的抗原 肾小球肾病，定位于肾包被窝 刷状缘上皮细胞，其功能尚不清楚。重点是 是关于确定结构和生物功能的 Gp330的同源性。目标是(A)确定完整的氨基酸 克隆人gp330基因序列，(B)鉴定gp330基因 特异性识别配体S的gp330的功能特性 在gp330高表达的肾上皮的背景下， (C)确定gp330在成体和胚胎中的表达模式 组织以及培养的细胞系中也是如此。这个项目的一个相关目标 是用来分离和鉴定LDLR家族的新成员 基因组同源-聚合酶链式反应克隆及简并寡核苷酸筛选 图书馆。