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STRUCTURE AND FUNCTION OF GP330

GP330的结构与功能

基本信息

批准号：
2144810
负责人：
WILLIAM SCOTT ARGRAVES
金额：
$ 12.65万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
起止时间：
1992-09-30 至 1998-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2144810
关键词：
affinity chromatography complementary DNA genetic library glycoprotein structure human tissue in situ hybridization laboratory mouse laboratory rabbit laboratory rat low density lipoprotein receptor molecular cloning polymerase chain reaction protein sequence protein structure function receptor binding receptor expression tissue /cell culture

项目摘要

The low density lipoprotein receptor (LDLR) is at the head of an emerging family of structurally and perhaps functionally related receptors. The family presently contains five members that include the LDLR, alpha2-macroglobulin receptor/low density lipoprotein receptor-related protein (alpha2MR/LRP), the 95 kDa avian oocyte-specific receptor for the yolk precursor vitellogenin, the avian 380 kDa ovarian follicle membrane protein, and the kidney proximal tubule membrane protein, glycoprotein 330 (gp330). Although gp330 has been implicated as the pathogenic antigen for Heymann nephritis, the rat model for membranous glomerulonephropathy, and it has been localized to coated-pits of kidney brush border epithelial cells, its function is not known. The emphasis of this proposal is on determining the structure and biological function of gp330. The objectives are to (a) determine the complete amino acid sequence of human gp330 by cDNA cloning, (b) to characterize the functional properties of gp330 by identifying its ligand(s) particularly in the context of renal epithelium where gp330 is highly expressed and, (c) to determine the expression pattern of gp330 in adult and embryonic tissues as well as in cultured cell lines. A related aim of this project is to isolate and characterize new members of the LDLR family using homology-PCR cloning and degenerate oligonucleotide screening of genomic libraries.

低密度脂蛋白受体（LDLR）是一种新兴的结构上和功能上可能相关的受体家族。的家族目前包含五个成员，包括LDLR， α 2-巨球蛋白受体/低密度脂蛋白受体相关蛋白（alpha 2 MR/LRP），95 kDa禽卵母细胞特异性受体，卵黄前体蛋白卵黄蛋白原，鸟类380 kDa卵泡膜蛋白质和肾近曲小管膜蛋白，糖蛋白 330（gp330）。尽管gp 330被认为是致病性的， Heymann肾炎抗原，膜性肾炎大鼠模型，肾小球肾病，并已定位于包被小凹的肾刷状缘上皮细胞，其功能尚不清楚。重点这一建议的重点是确定结构和生物功能关于GP 330 目的是（a）确定完整的氨基酸通过cDNA克隆的人gp 330的序列，（B）以表征通过鉴定它的配体，特别是在其中gp 330高度表达的肾上皮的情况下， (c)为了确定gp 330在成体和胚胎中的表达模式，组织以及培养的细胞系中。本项目的相关目标分离和鉴定LDLR家族的新成员，基因组同源PCR克隆和简并寡核苷酸筛选图书馆.