MECHANISM OF HORMONE-DEPENDENT GENE REGULATION IN RICE

水稻激素依赖性基因调控机制

• 批准号: 3300937
• 负责人: RAY J. WU
• 金额: $ 17.24万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3300937
• 关键词: DNA binding protein; amylases; gene expression; genetic promoter element; genetic regulatory element; genetically modified plants; hormone regulation /control mechanism; messenger RNA; molecular cloning; nucleic acid sequence; plant genetics; regulatory gene; rice; transcription factor

Our long term objective is to understand, at the molecular level, how the plant hormone gibberellin (GA) regulates gene expression in rice, a representative cereal system. The specific objectives: 1 . To define the exact DNA sequence(s) in the regulatory regions of alpha-amylase genes required for the in vivo GA stimulation of gene expression. 2. To define the exact DNA sequence(s) In the regulatory regions of alpha-amylase genes required for the in vitro binding of the GA-dependent DNA binding (GA-DB) protein(s) in rice and relate this to results of in vivo experiments. 3. To test if the GA-dependent DNA binding protein(s) are transcription factor(s). 4. To purify and characterize the GA-dependent DNA binding protein(s). This is indicated if elements essential for GA action in vivo bind the protein dependent on GA in vitro. 5. To clone the gene(s) that codes for the GA-dependent DNA binding protein(s) in rice. 6. To look for mRNAs representing immediate response genes after GA induction.

我们的长期目标是在分子水平上理解