MECHANISM OF HORMONE-DEPENDENT GENE REGULATION IN RICE

我们的长期目标是在分子水平上了解 植物激素赤霉素（GA）调节水稻的基因表达， 典型的谷物系统。 具体目标： 1.一、为了确定基因调控区的确切DNA序列， 基因的体内GA刺激所需的α-淀粉酶基因 表情 2.为了确定在调节区域中的确切DNA序列， α-淀粉酶基因需要在体外结合的GA依赖的 水稻中的DNA结合蛋白（GA-DB），并将其与 活体实验 3.为了测试GA依赖性DNA结合蛋白是否转录 因数。 4.纯化和表征GA依赖性DNA结合蛋白。 如果GA在体内的作用所必需的元件结合了 依赖于GA的蛋白质。 5.克隆编码GA依赖性DNA结合的基因 大米中的蛋白质。 6.寻找代表GA后立即反应基因的mRNA 诱导

Our long term objective is to understand, at the molecular level, how the plant hormone gibberellin (GA) regulates gene expression in rice, a representative cereal system. The specific objectives: 1 . To define the exact DNA sequence(s) in the regulatory regions of alpha-amylase genes required for the in vivo GA stimulation of gene expression. 2. To define the exact DNA sequence(s) In the regulatory regions of alpha-amylase genes required for the in vitro binding of the GA-dependent DNA binding (GA-DB) protein(s) in rice and relate this to results of in vivo experiments. 3. To test if the GA-dependent DNA binding protein(s) are transcription factor(s). 4. To purify and characterize the GA-dependent DNA binding protein(s). This is indicated if elements essential for GA action in vivo bind the protein dependent on GA in vitro. 5. To clone the gene(s) that codes for the GA-dependent DNA binding protein(s) in rice. 6. To look for mRNAs representing immediate response genes after GA induction.