MECHANISM OF HORMONE-DEPENDENT GENE REGULATION IN MICE

小鼠激素依赖性基因调控机制

• 批准号: 3300936
• 负责人: RAY J. WU
• 金额: $ 16.68万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3300936
• 关键词: DNA binding protein; amylases; gene expression; genetic promoter element; genetic regulatory element; genetically modified plants; hormone regulation /control mechanism; messenger RNA; molecular cloning; nucleic acid sequence; plant genetics; regulatory gene; rice; transcription factor

Our long term objective is to understand, at the molecular level, how the plant hormone gibberellin (GA) regulates gene expression in rice, a representative cereal system. The specific objectives: 1 . To define the exact DNA sequence(s) in the regulatory regions of alpha-amylase genes required for the in vivo GA stimulation of gene expression. 2. To define the exact DNA sequence(s) In the regulatory regions of alpha-amylase genes required for the in vitro binding of the GA-dependent DNA binding (GA-DB) protein(s) in rice and relate this to results of in vivo experiments. 3. To test if the GA-dependent DNA binding protein(s) are transcription factor(s). 4. To purify and characterize the GA-dependent DNA binding protein(s). This is indicated if elements essential for GA action in vivo bind the protein dependent on GA in vitro. 5. To clone the gene(s) that codes for the GA-dependent DNA binding protein(s) in rice. 6. To look for mRNAs representing immediate response genes after GA induction.

我们的长期目标是在分子水平上了解 植物激素赤霉素(GA)调节水稻的基因表达，a 代表性的谷物系统。 具体目标： 1.确定基因调控区的准确序列(S) 体内GA刺激基因所需的α-淀粉酶基因 表情。 2.确定基因调控区的准确序列(S) 依赖GA的α-淀粉酶基因体外结合所需 水稻中的DNA结合蛋白(GA-DB)(S)及其与In 活体实验。 3.检测GA依赖的DNA结合蛋白(S)是否转录 因素(S)。 4.纯化并鉴定GA依赖的DNA结合蛋白(S)。 这表明如果在体内GA作用所必需的元素结合 在体外依赖于GA的蛋白质。 5.克隆编码GA依赖的DNA结合的基因(S) 大米中的蛋白质(S)。 6.寻找GA后代表即时反应基因的mRNAs 归纳法。