小鼠激素依赖性基因调控机制-猫眼课题宝

小鼠激素依赖性基因调控机制

批准号：

3300936

负责人：

RAY J. WU

金额：

$16.68万

依托单位：

CORNELL UNIVERSITY

依托单位国家：

美国

项目类别：

财政年份：

1992

资助国家：

美国

项目状态：

已结题

起止时间：

1992-02-01 至 1995-01-31

来源：

https://reporter.nih.gov/project-details/3300936

关键词：

DNA binding protein amylases gene expression genetic promoter element genetic regulatory element genetically modified plants hormone regulation /control mechanism messenger RNA molecular cloning nucleic acid sequence plant genetics regulatory gene rice transcription factor

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中文摘要

翻译

我们的长期目标是在分子水平上理解

英文摘要

Our long term objective is to understand, at the molecular level, how the plant hormone gibberellin (GA) regulates gene expression in rice, a representative cereal system. The specific objectives: 1 . To define the exact DNA sequence(s) in the regulatory regions of alpha-amylase genes required for the in vivo GA stimulation of gene expression. 2. To define the exact DNA sequence(s) In the regulatory regions of alpha-amylase genes required for the in vitro binding of the GA-dependent DNA binding (GA-DB) protein(s) in rice and relate this to results of in vivo experiments. 3. To test if the GA-dependent DNA binding protein(s) are transcription factor(s). 4. To purify and characterize the GA-dependent DNA binding protein(s). This is indicated if elements essential for GA action in vivo bind the protein dependent on GA in vitro. 5. To clone the gene(s) that codes for the GA-dependent DNA binding protein(s) in rice. 6. To look for mRNAs representing immediate response genes after GA induction.

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