DNA SEQUENCE ANALYSIS OF EUCARYOTIC CYTOCHROME C GENES

真核细胞色素C基因的DNA序列分析

• 批准号: 3276701
• 负责人: RAY J. WU
• 金额: $ 29.57万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-04-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3276701
• 关键词: DNA; autoradiography; cytochrome c; endonuclease; genetic manipulation; genetic mapping; human tissue; molecular cloning; nucleic acid sequence; saltwater environment; wheat

The objectives of this proposed research are: (1) to clone the cytochrome c gene of human, monkey, rat, chicken, toad, fruit fly, clam, sea urchin and wheat; (2) to construct physical maps (using restriction enzymes) of the cytochrome c genes of the above organisms, including those of the coding region, 5' and 3' non-coding regions, and intron (if present); (3) to determine the DNA sequence of the entire cytochrome c gene of each of the above organisms; (4) to determine the DNA sequences of the 5' and 3' non-coding regions, and a portion of the introns (if present); and (5) to compare the DNA sequences obtained from the above organisms with one another and with the yeast cytochrome c gene.

本研究的目的是：（1）克隆细胞色素c基因 人、猴、大鼠、鸡、蟾蜍、果蝇、蛤、海胆和 小麦;（2）构建物理图谱（使用限制性内切酶）， 上述生物的细胞色素c基因，包括编码区的那些， 5'和3'非编码区，和内含子（如果存在）;（3）确定DNA 上述每种生物的整个细胞色素c基因的序列;（4） 确定5 ′和3 ′非编码区的DNA序列， 内含子（如果存在）;和（5）比较从 上述生物体彼此之间以及与酵母细胞色素c基因之间的相互作用。