MECHANISM OF HORMONE-DEPENDENT GENE REGULATION

激素依赖性基因调控机制

• 批准号: 2181360
• 负责人: RAY J. WU
• 金额: $ 18.04万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2181360
• 关键词: DNA binding protein; amylases; gene expression; genetic promoter element; genetic regulatory element; genetically modified plants; hormone regulation /control mechanism; messenger RNA; molecular cloning; nucleic acid sequence; plant genetics; regulatory gene; rice; transcription factor

Our long term objective is to understand, at the molecular level, how the plant hormone gibberellin (GA) regulates gene expression in rice, a representative cereal system. The specific objectives: 1 . To define the exact DNA sequence(s) in the regulatory regions of alpha-amylase genes required for the in vivo GA stimulation of gene expression. 2. To define the exact DNA sequence(s) In the regulatory regions of alpha-amylase genes required for the in vitro binding of the GA-dependent DNA binding (GA-DB) protein(s) in rice and relate this to results of in vivo experiments. 3. To test if the GA-dependent DNA binding protein(s) are transcription factor(s). 4. To purify and characterize the GA-dependent DNA binding protein(s). This is indicated if elements essential for GA action in vivo bind the protein dependent on GA in vitro. 5. To clone the gene(s) that codes for the GA-dependent DNA binding protein(s) in rice. 6. To look for mRNAs representing immediate response genes after GA induction.

我们的长期目标是在分子水平上了解如何 植物激素赤霉素（GA）调节水稻的基因表达， 具有代表性的谷物系统。 具体目标： 1.定义调控区域的精确 DNA 序列 体内 GA 刺激基因所需的 α-淀粉酶基因 表达。 2. 定义调控区域中的精确 DNA 序列 GA依赖性体外结合所需的α-淀粉酶基因 水稻中的 DNA 结合 (GA-DB) 蛋白，并将其与 in 的结果联系起来 体内实验。 3. 测试 GA 依赖性 DNA 结合蛋白是否转录 因素。 4. 纯化并表征 GA 依赖性 DNA 结合蛋白。 这表明体内 GA 作用所必需的元素是否与 体外依赖GA的蛋白质。 5. 克隆编码 GA 依赖性 DNA 结合的基因 大米中的蛋白质。 6. 寻找代表GA后立即反应基因的mRNA 就职。

项目成果

Functional dissection of a rice high-pI alpha-amylase gene promoter.

水稻高 pI α-淀粉酶基因启动子的功能剖析。

• DOI: 10.1007/bf00283513
• 发表时间: 1994
• 期刊: Molecular & general genetics : MGG
• 作者: Tanida,I;Kim,JK;Wu,R
• 通讯作者: Wu,R