PROTEINS, POLYPEPTIDES, AND AMINO ACIDS
蛋白质、多肽和氨基酸
基本信息
- 批准号:2183779
- 负责人:
- 金额:$ 14.73万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1992
- 资助国家:美国
- 起止时间:1992-12-01 至 1996-11-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
One of our long term interests has been the development of biocatalytic
methods for organic synthetic applications. More recently, we have been
interested in the utilization of enzymes to catalyze the modification of
complex molecules such as proteins and peptides (posttranslational
modification) by taking advantage of their chemoselective properties.
Consistent with this objective, we intend to focus our attention on three
specific projects: [1] To develop rapid sensitive methods for the
isolation and identification of the C-terminal fragment of proteins. The
first approach entails the enzymatic attachment of a fluorescent amino
acid derivative onto the C-terminus of a protein without side reactions;
the second method involves the selective epimerization of the C-terminal
amino acid. The protein is cleaved into peptide fragments and the non
C-terminal peptides are hydrolyzed with carboxypeptidases Y and B. The
C-terminal peptide is then isolated and sequenced by Edman chemistry or
by FAB mass spectrometry; the third approach takes advantage of the
kinetics of chymotryptic cleavage; the first non-ultraviolet light
absorbing peptide formed is the C-terminal fragment. [2] To purify and
characterize the enzymes present in bacteria that could selectively
deacylate N-terminal acetylated proteins and peptide hormones; the
unblocked proteins could then be sequenced by Edman chemistry. [3] To
develop a general chemoenzymatic asymmetric synthesis of amino acids.
This approach involves the lipase-catalyzed enantioselective hydrolysis
of 4-substituted-2-phenyl-oxazolin-5-ones to yield optically-active N-
benzoyl amino acids of either configuration.
我们的长期兴趣之一是生物催化的发展
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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CHARLES J SIH其他文献
CHARLES J SIH的其他文献
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{{ truncateString('CHARLES J SIH', 18)}}的其他基金
BIO-ORGANIC CHEMISTRY OF OXIDATIVE PHENOLIC COUPLING
氧化酚偶联的生物有机化学
- 批准号:
6381192 - 财政年份:1998
- 资助金额:
$ 14.73万 - 项目类别:
BIO-ORGANIC CHEMISTRY OF OXIDATIVE PHENOLIC COUPLING
氧化酚偶联的生物有机化学
- 批准号:
2670476 - 财政年份:1998
- 资助金额:
$ 14.73万 - 项目类别:
BIO-ORGANIC CHEMISTRY OF OXIDATIVE PHENOLIC COUPLING
氧化酚偶联的生物有机化学
- 批准号:
6177690 - 财政年份:1998
- 资助金额:
$ 14.73万 - 项目类别:
BIO-ORGANIC CHEMISTRY OF OXIDATIVE PHENOLIC COUPLING
氧化酚偶联的生物有机化学
- 批准号:
2906264 - 财政年份:1998
- 资助金额:
$ 14.73万 - 项目类别:
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