GENETIC LABORATORY

基因实验室

• 批准号: 2282913
• 负责人: WILLIAM H STONE
• 金额: $ 12.54万
• 依托单位: TRINITY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1995-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2282913
• 关键词: Macaca mulatta; Pan; Primates; adenosine deaminase; animal genetic material tag; animal population genetics; animal tissue; blood group antigens; blood groups; carbonate dehydratase; chemical fingerprinting; complement; dihydrolipoamide dehydrogenase; erythrocytes; gene frequency; genetic markers; genetic strain; genotype; glucose 6 phosphate isomerase; inbreeding; isozymes; mannose 6 phosphate isomerase; phosphogluconate dehydrogenase; tissue resource /registry; transferrin

A Genetics Laboratory for Typing Nonhuman Primates Nonhuman primates are excellent models of human biology and behavior because of their close relationship to humans and because there is now a large number of established genetic markers and an increasing availability of pedigreed colonies. This application is a frank request for base support of a service laboratory which will serve as a research resource for genetic typing of nonhuman primates. This laboratory will be equipped to type for more than 20 erythrocyte antigens (blood groups) of rhesus monkeys, two serum proteins (transferrins and C3 complement), and at least eight isozymes (multiple molecular forms of enzymes) in several different species. For example, in rhesus monkeys it will be able to provide typing for more than 18 different genetic systems which taken together can define more than a trillion different phenotypes in this species. We are also developing the technic of DNA typing (fingerprinting) which is the most powerful new tool for identification and parentage determination. This new ethnic will greatly expand our service capability to a wide variety of nonhuman primate species. These genetic markers are essential tool for maintaining accurate pedigree records; without them it would be impossible to resolve disputed paternity which is encountered often in breeding colonies. In addition, these markers will be used to maintain the accuracy of breeding records by detecting errors resulting from mistaken identity, improper transcriptions, infant switches or unexpected matings. Finally, these genetic markers will be used to monitor inbreeding and loss of genetic variability which can threaten the very survival of nonhuman primate populations.

非人类灵长类基因分型实验室 非人灵长类动物是研究人类生物学和行为的极好模型 因为它们与人类的关系很近， 大量已建立的遗传标记和越来越多的可用性 纯种的殖民地。 此应用程序是一个坦率的要求基地支持的服务 实验室，将作为一个研究资源的基因分型， 非人类灵长类动物 这个实验室将配备打字机， 20种恒河猴红细胞抗原（血型），两种血清 蛋白质（转铁蛋白和C3补体）和至少8种同工酶 （酶的多种分子形式）在几个不同的物种。 为 例如，在恒河猴中，它将能够提供超过 18种不同的遗传系统，可以定义超过一个 在这个物种中有万亿种不同的表型。 我们也在开发 DNA分型技术（指纹）是最强大的新工具 用于身份鉴定和亲子鉴定。 这个新的民族将 极大地扩展了我们对各种非人灵长类动物的服务能力 物种 这些遗传标记是维持准确的 家谱记录;没有它们，就不可能解决有争议的问题。 在繁殖群体中经常遇到的父子关系。 此外，本发明还提供了一种方法， 这些标记将用于保持育种记录的准确性， 检测由错误的身份，不正确的传输， 婴儿交换或意外交配。 最后，这些遗传标记将 用于监测近亲繁殖和遗传变异性的丧失， 威胁到非人类灵长类动物的生存