GENETIC MANAGEMENT OF CHIMPANZEE BREEDING AND RESEARCH

这项建议的总体目标是服务于黑猩猩的繁育和 研究计划(CBRP)通过提供全面的遗传管理。 为此，我们将证实所有CBRP黑猩猩的亲子关系 在5个群体中的每个群体中都有专门的繁殖队列，并根据经验 量化遗传变异性的水平。这些数据将被用作 为合理的育种计划奠定了基础。我们将使用DNA指纹技术 以确定亲子关系并监测血统。我们还会打字 对于多态遗传标记，包括同工酶、转铁蛋白、RFLP、 VNR和二核苷酸重复。标记数据将用于 计算表征每个群体的群体遗传参数 允许对遗传多样性进行估计。基因数据将会 为将非CBRP男性引入 现有的群体--作为加强基因库的育种者。他们还将 为选择特定的后代提供理由 代表过多的父母包括在专门的繁殖队列中。一个 该项目提供的服务的重要成果将是 确保维持遗传多样性的黑猩猩种群 被圈养，从而确保它们继续获得生物医学 目的。

The overall goal of this proposal is to serve the Chimpanzee Breeding and Research Program (CBRP) by providing comprehensive genetic management. To this end, we will corroborate parentage of all CBRP chimpanzees in the dedicated breeding cohorts at each of the 5 colonies and empirically quantify levels of genetic variability. These data will be used as a basis for a rational breeding program. We will use, DNA fingerprinting to ascertain parentage and to monitor the pedigrees. We will also type for polymorphic genetic markers, including isozymes, transferrins, RFLPs, VNTRs, and dinucleotide repeats. The marker data will be used to calculate population genetic parameters that characterize each colony and allow for an estimate of genetic diversity. The genetic data will provide guidelines for the introduction of nonCBRP males into the existing colonies-as breeders to enhance the gene pool. They will also provide the rationale for selecting specific offspring of over-represented parents to include in the dedicated breeding cohort. An important result of the service provided by this project will be to assure the maintenance of a genetically diverse population of chimpanzees in captivity and thus assure their continued availability for biomedical purposes.