GENETIC LABORATORY FOR TYPING NONHUMAN PRIMATES

非人灵长类基因分型实验室 非人灵长类动物是人类生物学和行为的优秀模型 因为它们与人类的关系密切，而且现在有一种 大量已建立的遗传标记和越来越多的可用性 有血统的殖民地。 此应用程序是对服务的基本支持的坦率请求 实验室，将作为基因分型的研究资源 非人灵长类动物。这个实验室的打字能力将超过 恒河猴红细胞抗原(血型)20份，血清2份 蛋白质(转铁蛋白和补体C3)，以及至少八种同工酶 (多种分子形式的酶)在几个不同的物种中。为 例如，在恒河猴身上，它将能够提供超过 18种不同的遗传系统加在一起可以定义超过一个 这个物种有上万亿种不同的表型。我们还在开发 DNA分型(指纹)技术是最强大的新工具 用于身份鉴定和亲子关系确定。这一新的民族意志 将我们的服务能力大大扩展到各种非人类灵长类动物 物种。这些遗传标记是保持准确性的必要工具 血统记录；没有它们，就不可能解决争议 在繁殖群体中经常遇到的亲子关系。此外, 这些标记将用于通过以下方式保持育种记录的准确性 检测由错误的身份、不正确的转录、 婴儿交换或意想不到的配对。最后，这些遗传标记将 用于监测近亲交配和遗传变异的丧失，这可以 威胁到非人类灵长类种群的生存。

A Genetics Laboratory for Typing Nonhuman Primates Nonhuman primates are excellent models of human biology and behavior because of their close relationship to humans and because there is now a large number of established genetic markers and an increasing availability of pedigreed colonies. This application is a frank request for base support of a service laboratory which will serve as a research resource for genetic typing of nonhuman primates. This laboratory will be equipped to type for more than 20 erythrocyte antigens (blood groups) of rhesus monkeys, two serum proteins (transferrins and C3 complement), and at least eight isozymes (multiple molecular forms of enzymes) in several different species. For example, in rhesus monkeys it will be able to provide typing for more than 18 different genetic systems which taken together can define more than a trillion different phenotypes in this species. We are also developing the technic of DNA typing (fingerprinting) which is the most powerful new tool for identification and parentage determination. This new ethnic will greatly expand our service capability to a wide variety of nonhuman primate species. These genetic markers are essential tool for maintaining accurate pedigree records; without them it would be impossible to resolve disputed paternity which is encountered often in breeding colonies. In addition, these markers will be used to maintain the accuracy of breeding records by detecting errors resulting from mistaken identity, improper transcriptions, infant switches or unexpected matings. Finally, these genetic markers will be used to monitor inbreeding and loss of genetic variability which can threaten the very survival of nonhuman primate populations.