EXCITATORY AMINO ACIDS AND CORTEX DEVELOPMENT

这项研究的长期目标是了解 兴奋性氨基酸递质在皮质形态发生中的作用 和可塑性。介导EaaS活动的受体是 存在于发育中的大脑中，并已被证明在 学习和记忆模型以及发育可塑性。这个 拟议中的实验将检验EAA受体在 鼠桶状野皮质的正常发育及其在大脑皮层发育中的作用 新生儿外周血后发生的皮质结构重新分配 去感觉神经。具体目标是：1)描述个体发育的特征 胡须(外周输入)到Barrel(皮层)的EAA受体的数量 途径，2)确定新生儿是否引起EaaS的变化 给予EAA受体拮抗剂将改变桶的形成， 3)确定新生儿是否给予5-羟色胺能 神经毒素，PCA，延缓EAA受体的表达 晶须到桶的途径，4)确定新生儿的晶须 去掉大脑皮质的传入，将改变EAA的个体发育。 受体和5)以确定EAA对EAA系统的修饰 受体拮抗剂将改变细胞的结构重新分配和 通常发生在去传入之后的传入。EAA受体 将通过体外受体放射自显影和 免疫细胞化学。EAA拮抗剂和5-羟色胺的后果 神经毒素治疗和去除胡须对桶形成的影响将是 根据大小和细胞结构的变化，以模式进行评估 丘脑和非丘脑(5-羟色胺)传入的密度和密度 EAA受体的分布。 发育中的晶须到桶的途径将作为 考察EaaS在发生的重组变化中的作用 在外周损伤后。因为感官信息来自于 外围设备很可能是由EaaS传达的， EAA受体在中枢神经系统外周地形图建立中的作用 皮质具有潜在的临床重要性。通过理解 神经递质对MAP形成的控制，有可能设计 促进受伤后恢复的策略。

The long term objective of this research is to understand the role that excitatory amino acid transmitters (EAAs) play in cortical morphogenesis and plasticity. The receptors that mediate the actions of EAAs are present in the developing brain and have been shown to play a role in models of learning and memory and in developmental plasticity. The proposed experiments will examine the role of EAA receptors in the normal development of rodent barrel field cortex and their role in the structural reassignment of cortex which occurs after neonatal peripheral deafferentation. The specific aims are 1) to characterize the ontogeny of EAA receptors in the whisker (peripheral input) to barrel (cortex) pathway, 2) to determine whether changes in EAAs caused by neonatal administration of EAA receptor antagonists will alter barrel formation, 3) to determine whether neonatal administration of the serotonergic neurotoxin, PCA, delays the development of EAA receptor expression in the whisker to barrel pathway, 4) to determine whether neonatal whisker removal, which deafferents the cortex, will alter the ontogeny of EAA receptors and 5) to determine whether modification of EAA systems by EAA receptor antagonists will alter the structural reassignment of cells and afferents which normally occurs after deafferentation. EAA receptors will be visualized by in vitro receptor autoradiography and immunocytochemistry. The consequences of EAA antagonists and 5HT neurotoxin treatments and of whisker removal on barrel formation will be assessed in terms of changes in size and cytoarchitecture, in patterns of thalamic and non-thalamic (5-HT) afferents and in the density and distribution of EAA receptors. The developing whisker to barrel pathway will serve as a model for examining the role of EAAs in the reorganizational changes which occur after peripheral injury. Since sensory information from the peripheriphery is likely conveyed by EAAs, the characterization of the role of EAA receptors in the establishment of peripheral maps in the cortex is potentially clinically important. By understanding the neurotransmitter control of map formation, it may be possible to design strategies to enhance recovery after injury.