UTERINE METABOLISM AND BODY TEMPERATURE DURING DECIDUALIZED PSEUDOPREGNANCY

蜕膜假妊娠期间的子宫代谢和体温

• 批准号: 5225744
• 负责人: FITZGERALD SPENCER
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5225744
• 关键词: albino rat; body temperature; decidua; dexamethasone; dosage; drug adverse effect; glycogen; medical education; minority institution research support; progesterone; progesterone receptors; protein metabolism; pseudopregnancy; telecommunications; uterus

The objective of this study will be to investigate whether dexamethasone (DEX), a synthetic glucocorticoid useful in chemical reproductive therapy in humans, can adversely affect two functions: namely, (1) cellular aspects of the uterine metabolism during decidualized pseudopregnancy (DCPG), thereby constituting a basis for the interruption of pseudopregnancy, and (2) the body temperature rhythm during this reproductive state. Biochemical endpoints (uterine weight, protein, glycogen, progesterone, cytosolic and nuclear progesterone receptors, and plasma progesterone) during deciduoma formation on Day 10 DCPG will be investigated to help clarify the possible mechanism of DEX action. The body temperature on Days 10-12 DCPG will be studied in order to evaluate the influence of DEX on this general function. The albino rat will be employed as the mammalian model. Rats, between 200-250 grams, will be maintained under controlled photoperiodic conditions of 12L:12D (lights on at 0500 hr., off at (700 hr.) and of ambient temperature (21 degrees +/- 2 degrees C). Those rates displaying at least two consistent 4 or 5 days estrous cycle (determined by vaginal lavages) will be used. After cervical stimulation with a vibrating plastic rod during proestrus and estrus, rats will undergo the induction of uterine deciduoma on the 4th day of continuous leucocytic infiltration in the vaginal lavages. This procedure will be surgically performed via laparotomy and by scratching the inner anti-mesometrial uterine membrane with a specially designed DeFeo needle. During the post-operative recovery period, rats, divided into three groups, will be subcutaneously injected with DEX (0.5,1.0 and 1.5 mg/rat) in daily doses for 5 days (Days 5-9 DCPG). Injections will occur at 0700 hr. (2 hours after the dark phase, and biochemical tests performed during the mid-light phase at 1100 hr. on Day 10 DCPG), and at 1500 hr. (2 hours before the dark phase, and biochemical tests conducted during the mid-dark phase at 2300 hr. on Day 10 DCPG). The body temperature will be continuously monitored and analyzed by means of a computerized biotelemetry system (Dataquest III) during Days 9-10 DCPG. Each group will consist of 10 animals. Results presented as means +/- standard errors, will be tested statistically, with a p value of a least 0.05 considered significant. Dose-response, Student's t, Analysis of Variance and the Cosinor regression methods will be employed for analyses of the data.

本研究的目的是调查地塞米松是否 (DEX)，一种用于化学生殖治疗的合成糖皮质激素 在人类中，可能对两种功能产生不利影响：即（1）细胞 蜕膜化假妊娠期间子宫代谢的各个方面 （DCPG），从而构成中断的基础 （2）在此期间的体温节律 生殖状态生化终点（子宫重量，蛋白质， 糖原、孕酮、胞质和核孕酮受体，和 DCPG第10天蜕膜瘤形成期间的血浆孕酮）将被 研究有助于澄清DEX作用的可能机制。的 将研究DCPG第10-12天的体温，以评价 DEX对这个功能的影响。 这只白化病鼠 作为哺乳动物模型。体重在200-250克之间的老鼠， 在12 L：12 D的受控光周期条件下维持（开灯 在0500小时，关闭（700小时）和环境温度（21度+/- 2摄氏度）。 这些利率显示至少两个一致的4或5天 将使用发情周期（通过阴道灌洗确定）。宫颈 在发情前期和发情期用振动塑料棒刺激，大鼠 将在第4天进行子宫蜕膜瘤诱导， 阴道灌洗液中持续的白细胞浸润。本程序 将通过剖腹手术进行，并通过刮擦内部 使用专门设计的DeFeo针对子宫系膜进行穿刺。 在术后恢复期，将大鼠分为三组， DEX（0.5、1.0和1.5mg/只）皮下注射 每日给药5天（第5-9天DCPG）。 注射将在0700进行 HR.（黑暗阶段后2小时，在黑暗阶段期间进行生化测试） 第10天DCPG 1100小时的中光照期）和1500小时（2小时 在黑暗阶段之前，在黑暗中期进行生化测试， 在第10天DCPG的2300小时阶段）。体温会 连续监测和分析，通过计算机化的 在DCPG第9-10天期间使用生物遥测系统（Dataquest III）。每组将 10只动物。结果表示为平均值+/-标准误， 将进行统计学检验，认为p值至少为0.05 显著剂量反应、学生t检验、方差分析和 将采用余弦回归方法分析数据。