NEURAL PLASTICITY IN PELVIC VISCERAL TISSUES

盆腔内脏组织的神经可塑性

• 批准号: 2263678
• 负责人: WILLIAM G DAIL
• 金额: $ 11.84万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-07-01 至 1997-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2263678
• 关键词: autonomic nervous system; axon; biomarker; dendrites; electron microscopy; histochemistry /cytochemistry; immunocytochemistry; innervation; laboratory rat; microtubule associated protein; neural growth associated protein; neural plasticity; neurotransmitters; nitric oxide; penis erection; postganglionic fiber; preganglionic fiber; protooncogene; spinal cord injury; splanchnic nerves; synapses; vasodilation; vasodilators

There is sufficient reason to believe that the full consideration of complications following injury of the nervous system should include synaptic reorganization, in addition to the loss of specific neural elements. Synaptic plasticity may occur at varied levels in the neural pathways, which for visceral tissues such as the penis, include the central nervous system, autonomic ganglia and peripheral target tissues. Stimuli for reorganization in autonomic ganglia may be especially vigorous, perhaps muting or at least influencing the course of change at other levels. By documenting specific plastic changes in pelvic autonomic pathways, the proposed studies are the first attempt to define the milieu in the pelvic plexus subsequent to injury. Specifically, the changing synaptology of penile parasympathetic neurons will be followed after their partial or total disconnection from the spinal cord. These studies model injury to the sacral portion of the spinal cord, including that represented by direct trauma to the conus medullaris and cauda equina or by conditions such as spina bifida. It is proposed to: (1) use quantitative electron microscopy to describe the synaptology of postganglionic penile neurons following interruption of preganglionic nerves, (2) employ histochemistry and immunohistochemistry for neurotransmitters/neuromodulators to determine whether penile neurons reacquire a specific subset of presynaptic fibers, (3) analyze the contribution of dendrites and axons to the emergent innervation of decentralized penile neurons by staining for microtubular associated protein (MAP-2) and growth associated protein (GAP-43), (4) analyze changes in the neural circuitry in pelvic ganglia following nerve lesions by using c-fos immunoreactivity as a metabolic marker, (5) to determine whether hypogastric nerve-induced erection, apparent after nerve lesions, is mediated by nitric oxide, (6) to determine whether the post-lesion, plastic changes in the innervation of the corpus cavernosum also occurs in the other erectile body of the penis, the corpus spongiosum. These studies should unravel the degree to which the ordered arrangement of pre- and postganglionic connections is altered by nerve injury. This knowledge is central to understanding perturbations in control of visceral organs and the application of strategies to improve function.

有充分的理由相信，充分考虑 神经系统损伤后的并发症应该包括 突触重组，以及特定神经的丧失 元素。突触可塑性可能发生在神经中不同的水平。 内脏组织(如阴茎)的通路包括 中枢神经系统、自主神经节和周围靶组织。 对自主神经节重组的刺激可能特别 充满活力的，也许是沉默的，或者至少影响着变化的进程 其他层面。通过记录骨盆的特定整形变化 自主神经通路，拟议的研究是第一次尝试定义 骨盆神经丛受伤后的周围环境。具体地说， 接下来将观察阴茎副交感神经元突触的变化 在它们与脊髓部分或全部断开后。这些 研究脊髓骶部损伤的模型，包括 表现为脊髓圆锥和尾部的直接创伤 或由脊柱裂等情况引起的。建议：(1) 用定量电子显微镜描述海马神经元的突触 节前切断后的阴茎节后神经元 神经，(2)采用组织化学和免疫组织化学方法 神经递质/神经调质决定阴茎神经元 重新获取特定的突触前纤维子集，(3)分析 树突和轴突在突起神经支配中的作用 微管相关染色显示分散的阴茎神经元 蛋白质(MAP-2)和生长相关蛋白(GAP-43)，(4)分析 神经损伤后盆神经节内神经回路的变化 以c-fos免疫反应作为代谢标记物，(5)确定 无论是腹下神经引起的勃起，在神经损伤后明显， 是由一氧化氮介导的，(6)确定损伤后， 海绵体的神经支配也会发生可塑性变化 在阴茎的另一个可勃起的身体中，海绵体。这些 研究应该在多大程度上揭示有序的安排 神经节前和节后的连接会因神经损伤而改变。这 知识是理解微扰控制的核心。 脏腑功能的改善和应用策略的应用。