STUDY OF INNERVATION OF TISSUE

最近的一个认识是， 阴茎勃起有着有机的基础，一直备受关注， 神经和血管基础知识中尚未解决的问题 正常勃起的情况。 阴茎勃起的复杂性 强调了相关的解剖和功能 在一个良好的模型中进行研究。 血液变化 在勃起过程中阴茎中的分布尚不清楚， 也不确定如何主动（神经肌肉和被动机制 有助于勃起。 评估阴茎重要部位 在调节血液流动，并获得全面的数据， 阴茎海绵体和阴茎体的脉管系统 海绵体 血管铸型的扫描电子显微镜检查将 阴茎在松弛状态和刺激后， 血管扩张神经 自主神经的神经组织化学研究 将进行孢子体的神经支配， 了解这个勃起的身体如何有助于阴茎 勃起 有人怀疑阴茎血管扩张神经 是胆碱能的，因此阴茎光滑的器官浴研究 肌肉将被用来确定内源性物质，如 乙酰胆碱引起血管扩张。 较新的胆碱能激动剂和 拮抗剂药物将用于受体放射自显影 研究和器官浴准备，以评估前和 乙酰胆碱的突触后效应。 参与 阴茎勃起中的腹下神经尚不清楚，但它是 很明显，它可以补偿主要的血管扩张途径， 骶骨起源 神经刺激和压力测量 阴茎将被用于（1）确定附件是否 血管扩张通路与骶神经通路协同勃起 （2）随时间推移进行随访并定量 神经损伤后血管舒张通路的代偿性变化。 这些研究旨在提供基本的 解释阴茎机制所需的信息 正常和病理状态下的勃起。

The rather recent realization that many of the disturbances of penile erection have an organic basis, has focused attention on unresolved problems in basic knowledge of the neural and vascular events in normal erection. The complexity of penile erection underscores the need for correlative anatomical and functional studies in a well-characterized model. Changes in blood distribution in the penis during erection are not well-understood, nor is it certain how active (neuro-muscular and passive mechanisms contribute to erection. To evaluate sites in the penis important in regulating blood flow and to obtain comprehensive data on the vasculature of the corpora cavernosa penis and the corpus spongiosum. Scanning electron microscopy of vascular casts will be made of the penis in the fiaccid state and after stimulation of vasodilator nerves. Neurohistochemical studies of the autonomic innervation of the corpus sporgiosum will be performed to understand how this erectile body may contribute to penile erection. Doubt has been expressed that penile vasodilator nerves are cholinergic, therefore organ bath studies of penile smooth muscle will be used to determine how endogenous substances such as acetylcholine cause vasodilation. Newer cholinergic agonists and antagonists drugs will be used both in receptor autoradiographic studies and in organ bath preparations to evaluate pre- and postsynaptic effects of acetylcholine. Participation of the hypogastric nerve in penile erection is not understood, but it is clear that it may compensate for the main vasodilator pathway of sacral origin. Nerve stimulation and measurement of pressure in the penis will be used (1) to determine if the accessory vasodilator pathway cooperates with the sacral pathway in erection in the intact animal and (2) to follow over time and quantitate compensatory changes in the vasodilator pathway after nerve injury. The proposed studies are intended to provide fundamental information necessary for interpreting the mechanism of penile erection in the normal and pathological state.