STUDY OF INNERVATION OF TISSUE

组织神经支配的研究

• 批准号: 3399928
• 负责人: WILLIAM G DAIL
• 金额: $ 9.63万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-07-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3399928
• 关键词: acetylcholine; acetylcholinesterase; autonomic nervous system; autoradiography; catecholamines; electron microscopy; electrostimulus; fluorescence; ganglions; histochemistry /cytochemistry; immunochemistry; innervation; laboratory rat; mathematical model; microscopy; muscle contraction; muscle relaxants; neuroanatomy; neurochemistry; neurofilament; neurophysiology; neurotransmitters; norepinephrine; parasympathetic nervous system; penis; penis disorder; peripheral nervous system; smooth muscle; spinal cord; spinal cord injury; sympathetic nervous system; synapses; vasoactive intestinal peptide; vasodilation

The rather recent realization that many of the disturbances of penile erection have an organic basis, has focused attention on unresolved problems in basic knowledge of the neural and vascular events in normal erection. The complexity of penile erection underscores the need for correlative anatomical and functional studies in a well-characterized model. Changes in blood distribution in the penis during erection are not well-understood, nor is it certain how active (neuro-muscular and passive mechanisms contribute to erection. To evaluate sites in the penis important in regulating blood flow and to obtain comprehensive data on the vasculature of the corpora cavernosa penis and the corpus spongiosum. Scanning electron microscopy of vascular casts will be made of the penis in the fiaccid state and after stimulation of vasodilator nerves. Neurohistochemical studies of the autonomic innervation of the corpus sporgiosum will be performed to understand how this erectile body may contribute to penile erection. Doubt has been expressed that penile vasodilator nerves are cholinergic, therefore organ bath studies of penile smooth muscle will be used to determine how endogenous substances such as acetylcholine cause vasodilation. Newer cholinergic agonists and antagonists drugs will be used both in receptor autoradiographic studies and in organ bath preparations to evaluate pre- and postsynaptic effects of acetylcholine. Participation of the hypogastric nerve in penile erection is not understood, but it is clear that it may compensate for the main vasodilator pathway of sacral origin. Nerve stimulation and measurement of pressure in the penis will be used (1) to determine if the accessory vasodilator pathway cooperates with the sacral pathway in erection in the intact animal and (2) to follow over time and quantitate compensatory changes in the vasodilator pathway after nerve injury. The proposed studies are intended to provide fundamental information necessary for interpreting the mechanism of penile erection in the normal and pathological state.

最近人们意识到，许多骚乱 阴茎勃起有其有机基础，一直备受关注 神经和血管基础知识中尚未解决的问题 正常勃起时发生的事件。阴茎勃起的复杂性 强调了相关解剖和功能的必要性 在一个特征明确的模型中进行研究。血液中的变化 勃起过程中阴茎的分布还不是很清楚， 也不确定有多活跃(神经-肌肉和被动机制 有助于勃起。评估阴茎中的部位很重要 以调节血液流动，并获得有关 阴茎海绵体和阴茎体部的血管构筑 海绵体。血管铸型的扫描电子显微镜将 由处于僵硬状态的阴茎制成，并在刺激 血管扩张神经。自主神经的神经组织化学研究 将对孢子体进行神经支配以 了解这种勃起的身体可能对阴茎有何贡献 勃起。有人怀疑阴茎血管扩张神经 是胆碱能的，因此器官浴对阴茎光滑的研究 肌肉将被用来确定内源性物质如 乙酰胆碱会导致血管扩张。新的胆碱能激动剂和 受体放射自显影中将使用拮抗剂药物 研究和在器官浴制剂中评价前和 乙酰胆碱的突触后效应。中国政府的参与 腹下神经在阴茎勃起中的作用尚不清楚，但它是 明确它可能补偿血管扩张的主要途径 起源于骶骨。神经刺激与压力测量 阴茎将被用来(1)确定附件是否 血管扩张途径与骶骨途径在勃起中的协同作用 在完整的动物身上，(2)随着时间的推移和定量 神经损伤后血管扩张途径的代偿性改变。 拟议的研究旨在提供基本的 解释阴茎机制所需的信息 勃起处于正常和病理状态。