DVR--VISUAL PATHWAYS AND THE ORIGINS OF NEOCORTEX

DVR--视觉通路和新皮质的起源

• 批准号: 2265282
• 负责人: HARVEY Jules KARTEN
• 金额: $ 24.74万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1995-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2265282
• 关键词: chick embryo; electron microscopy; embryo /fetus; evolution; immunocytochemistry; in situ hybridization; laboratory rabbit; monoclonal antibody; neocortex; neuroanatomy; neuropeptides; neurotransmitters; pigeons; receptor; sensorimotor system; telencephalon; visual pathways

The mammalian neocortex is the product of a complex evolutionary history. Research on the organization of sensory projections to the telencephalon of nonmammalian vertebrates has demonstrated the presence of cortical equivalent "cluster" of neurons within the Dorsal Ventricular Ridge (DVR) of the telencephalon. These "clonal clusters" may correspond to individual laminae of the mammalian neocortex. The microcircuitry of connections between "clusters" within the tectofugal pathways appears to correspond to the interlaminar organization of extrastriate visual pathways of the neocortex. Further analysis of this pathway will help clarify the role of the extrastriate system in visual performance. Studies will concentrate on the organization of visual pathways within the DVR, the relationship of the DVR to the neocortex, and possible role of the subventricular zone of Stensaas in the ontogeny of neocortex in mammals. The DVR may correspond to the Subventricular Zone (SVZ) of Stensaas in the developing neocortex of mammals. Specific studies will include: A. Analysis of the organization, histochemistry and morphology of the neurons of the tectofugal pathway in the DVR of birds. B. Interrelationship of the thalamofugal and tectofugal pathways. C. Production of monoclonal antibodies to identify "clonal clusters" and their possible cross reactivity with single laminae of the neocortex of mammals. E. Investigate the role of the SVZ of mammals as a possible representative of the Dorsal Ventricular Ridge, and its participation in the development of neocortex. These studies will clarify our understanding of the role of tectofugal pathways in vision, the evolutionary origins of the mammalian neocortex, and both normal and abnormal developmental events in the ontogeny of the mammalian neocortex. Methods to be employed include pathway tracing methods, single cell filling, immunohistochemistry, monoclonal antibodies and in situ hybridization to identify the transmitters/peptides, receptors and related proteins mediating organization and communication in these two pathways.

哺乳动物的大脑皮层是一个复杂的进化过程的产物， 历史 感觉投射组织的研究 非哺乳类脊椎动物的端脑已经证明了 存在皮质等效的神经元“集群”内的 端脑的背侧脑室脊（DVR）。 这些“克隆 集群”可以对应于哺乳动物的单个板层 新皮层 “集群”之间连接的微电路 在离顶盖通路内似乎对应于 纹状体外视路的层间组织 新皮层 对这一途径的进一步分析将有助于阐明 外纹系统在视觉表现中的作用。 研究 将集中在视觉通路的组织内， DVR，DVR与新皮层的关系， Stensaas脑室下区在个体发育中的作用 哺乳动物的大脑皮层 DVR可以对应于心室下室壁， 哺乳动物发育中的新皮层中的Stensaas区（SVZ）。 具体研究将包括： A. 组织学、组织化学和形态学分析 鸟类DVR中的离顶盖通路神经元。 B。 离丘脑通路和离顶盖通路的相互关系。 C. 生产单克隆抗体以鉴定“克隆 团簇”及其与单个纹层可能的交叉反应性 哺乳动物的大脑皮层。 E. 研究哺乳动物SVZ的作用， 背侧脑室脊的代表，及其 参与新皮层的发育。 这些研究将澄清我们对 视觉中的离顶盖通路， 哺乳动物的新皮层，以及正常和异常的发育 哺乳动物新皮层个体发育中的事件。 所采用的方法包括途径追踪方法、单细胞方法、免疫细胞化学方法和免疫细胞化学方法。 填充，免疫组织化学，单克隆抗体和原位 杂交以鉴定递质/肽、受体和 相关的蛋白质介导的组织和通信，在这些 两条路。