LAMINATION OF IPL AND TARGET SPECIFIC GANGLION CELLS

内皮层神经节细胞树突的层状结构 网状层（IPL）是其响应特性的主要决定因素。 不同的神经节细胞亚群具有独特的树突状形态， 从无长突细胞和双极细胞接收确定的传入连接， 靶向特定的中枢靶点（“靶向特定的神经节细胞”）。 它们对中心目标的作用是由 神经递质/调节剂。 使用免疫组织化学方法，我们有 证明了存在不同的化学特异性种群， 无长突细胞存在于所有脊椎动物中。 最近， 发现在青蛙的不同亚群中存在四种主要的肽 视网膜神经节细胞 每个神经节细胞亚群终止于单独的 中心目标。 本项目将研究层状组织和相互作用， 哺乳动物和鸟类IPL内的无长突细胞和神经节细胞。 的 项目包括三个部分： I.化学定义的无长突和神经节群体的贡献 在IPL中进行层压。 二.视网膜神经节细胞的树突状形态与递质 终止于视顶盖 三.神经元无长突细胞递质特异性亚群的鉴定 接收来自大脑的离心投射。 这些研究将有助于识别神经节细胞递质， 我们对视网膜的基本微电路的理解 organization. 使用的方法包括IHC、cDNA原位杂交、单细胞杂交、免疫组化和免疫组化。 充填法、EM法和路径追踪法。

The laminar organization of ganglion cell dendrites within the inner plexiform layer (IPL) is a major determinant of their response properties. Distinct subsets of ganglion cells possess unique dendritic morphologies, receive defined afferent connections from amacrine and bipolar cells, and project upon specific central targets ("Target Specific Ganglion Cells"). Their action on the central target is modulated by their neurotransmitters/modulators. Using immunohistochemical methods, we have demonstrated the existence of diverse populations of chemically specific amacrine cells in all classes of vertebrates. Most recently we have discovered the presence of four major peptides in distinct subsets of frog retinal ganglion cells. Each ganglion cell subset terminates in separate central targets. This project will study the laminar organization and interaction of amacrine cells and ganglion cells within the IPL of mammals and birds. The project consists of three parts: I. Contributions of chemically defined populations of amacrine and ganglion cells to lamination in the IPL. II. Dendritic morphology and transmitters of retinal ganglion cells terminating in the optic tectum. III. Identification of transmitter specific subsets of amacrine cells in receipt of centrifugal projections from the brain. These studies will help identify ganglion cell transmitters, and contribute to our understanding of the basic microcircuitry of the retinal organization. Methods to be used include IHC, cDNA in situ hybridization, single cell filling, EM and pathway tracing methods.