ANGIOGENESIS AND THE ROLE OF XEGR1 IN XENOPUS

描述（改编自申请人的摘要）：为了维持增长 和分化，胚胎必须创造一个功能性的循环系统， 在早期发展中。 关于组织间的相互作用， 其可能是诱导成血管细胞（血管）形成所需的 内皮前体）。 调查人员建议首先检查 表达早幼粒细胞的空间和时间表达模式 血管内皮特异性标记物xEGR 1（一种内皮素G蛋白 偶联受体），和一个假定的受体酪氨酸激酶同源物 血管特异性小鼠基因flk-1和flt。 她还建议， 确定青蛙胚胎中产生成血管细胞的区域 通过创建一个精确的细胞命运图，后来表达xEGR 1， 进一步检查发育中的血管组织的外观， 扫描电镜 第二，她建议检查组织 在成血管细胞诱导中可能重要的相互作用， 组织共培养和用不同的方法处理未确定的组织 已知的中胚层诱导剂或已知破坏中胚层的试剂 规范. 最后，她建议研究xEGR 1是否具有 在血管生成/血管发生中的直接作用， xEGR 1的表达以及用激动剂和拮抗剂处理细胞 xEGR 1蛋白

DESCRIPTION (Adapted from the applicant's abstract): To sustain growth and differentiation, embryos must create a functional circulatory system during early development. Little is known about the tissue interactions that may be required to induce the formation of angioblasts (vessel endothelial precursors). The investigator proposes first to examine the spatial and temporal expression pattern for cells that express an early vascular endothelium-specific marker, xEGR1 (an Endothelium G-Protein coupled Receptor), and a putative receptor tyrosine kinase homolog of vascular-specific mouse genes flk-1 and flt. She also proposes to determine the region of the frog embryo that gives rise to angioblasts by creating a precise fate map of cells that later express xEGR1, and further to examine the appearance of developing vacular tissue with scanning electron microscopy. Second, she proposes to examine tissue interactions that may be important in angioblast induction by using tissue co-cultures and treatment of undetermined tissues with various known mesoderm inducers or agents known to disrupt mesoderm specification. Finally, she proposes to investigate whether xEGR1 has a direct role in angiogenesis/vasculogenesis by forcing ectopic expression of xEGR1 and by treating cells with agonists and antagonists of xEGR1 protein.