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ANGIOGENESIS AND THE ROLE OF XEGR1 IN XENOPUS

爪蟾中的血管生成和 XEGR1 的作用

基本信息

批准号：
2205005
负责人：
MARGARET S SAHA
金额：
$ 10.43万
依托单位：
COLLEGE OF WILLIAM AND MARY
依托单位国家：
美国
项目类别：
财政年份：
1995
资助国家：
美国
起止时间：
1995-06-01 至 1999-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2205005
关键词：
G protein Xenopus angiogenesis angiogenesis factor antisense nucleic acid cell differentiation early embryonic stage gene expression in situ hybridization mixed tissue /cell culture protein tyrosine kinase receptor coupling receptor expression scanning electron microscopy vascular endothelium

项目摘要

DESCRIPTION (Adapted from the applicant's abstract): To sustain growth and differentiation, embryos must create a functional circulatory system during early development. Little is known about the tissue interactions that may be required to induce the formation of angioblasts (vessel endothelial precursors). The investigator proposes first to examine the spatial and temporal expression pattern for cells that express an early vascular endothelium-specific marker, xEGR1 (an Endothelium G-Protein coupled Receptor), and a putative receptor tyrosine kinase homolog of vascular-specific mouse genes flk-1 and flt. She also proposes to determine the region of the frog embryo that gives rise to angioblasts by creating a precise fate map of cells that later express xEGR1, and further to examine the appearance of developing vacular tissue with scanning electron microscopy. Second, she proposes to examine tissue interactions that may be important in angioblast induction by using tissue co-cultures and treatment of undetermined tissues with various known mesoderm inducers or agents known to disrupt mesoderm specification. Finally, she proposes to investigate whether xEGR1 has a direct role in angiogenesis/vasculogenesis by forcing ectopic expression of xEGR1 and by treating cells with agonists and antagonists of xEGR1 protein.

描述(改编自申请者的摘要)：可持续发展和分化，胚胎必须创造一个有功能的循环系统在早期发育过程中。人们对组织间的相互作用知之甚少可能需要诱导血管母细胞(血管)的形成内皮前体)。调查员建议首先检查表达早期基因的细胞的时空表达模式血管内皮细胞特异性标记物xEGR1(一种内皮G蛋白偶联受体)，以及一个可能的受体酪氨酸激酶同源物小鼠血管特异性基因flk-1和flt。她还提议确定青蛙胚胎中产生血管母细胞的区域通过创建后来表达xEGR1的细胞的精确命运图，以及进一步检查发育中的血管组织的外观。扫描电子显微镜。其次，她建议检查组织在血管母细胞诱导中可能很重要的相互作用组织共培养与不同类型待定组织的治疗已知的破坏中胚层的中胚层诱导剂或制剂规格。最后，她建议调查xEGR1是否具有通过强迫异位在血管生成/血管生成中的直接作用 XEGR1的表达及激动剂和拮抗剂对细胞的影响 XEGR1蛋白的表达。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Tissue-specific developmental expression of OAX, a Xenopus repetitive element.

OAX（非洲爪蟾重复元件）的组织特异性发育表达。

DOI：
10.1016/s0925-4773(00)00307-5
发表时间：
2000
期刊：
Mechanisms of development
影响因子：
2.6
作者：
Whitford,KL;Oakes,JA;Scholnick,J;Saha,MS
通讯作者：
Saha,MS

Short upstream region drives dynamic expression of hypoxia-inducible factor 1alpha during Xenopus development.

短上游区域驱动爪蟾发育过程中缺氧诱导因子 1α 的动态表达。

DOI：
10.1002/dvdy.20049
发表时间：
2004
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists.
影响因子：
0
作者：
Sipe,ConorW;Gruber,ErikaJ;Saha,MargaretS
通讯作者：
Saha,MargaretS

Elucidating the origins of the vascular system: a fate map of the vascular endothelial and red blood cell lineages in Xenopus laevis.

阐明血管系统的起源：非洲爪蟾血管内皮细胞和红细胞谱系的命运图。