PRECHRONIC & CHRONIC TOXICITY OF EMODIN

前纪

• 批准号: 2311021
• 负责人: J D PREJEAN
• 金额: $ 40万
• 依托单位: SOUTHERN RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-03-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2311021
• 关键词: PRECHRONIC; CHRONIC; TOXICITY; EMODIN

The specific objectives of this investigation is to evaluate the prechronic and chronic toxicity and carcinogenicity of emodin in Fischer-344 rats and B6C3F1 mice. The following studies will be performed: Fourteen-Day Repeated-Dose Prechronic Toxicity Study: This study will characterize the toxicity associated with dietary exposure to emodin including the identification of target organs, determination of a no-effect level (if feasible), differences in sensitivity between sexes and species, and the slope of the dose-response curve. Thirteen-Week (90-Day) Subchronic Study: In addition to obtaining toxicological data as described under the 14-day repeated-dose toxicity study, this study will also help to determine the doses for each strain and species in the chronic 2-year toxicity study. This investigation includes necropsy and histopathologic evaluations, preparation of unstained blood smears (for use by the NTP in micronuleic determinations) and determination of blood concentrations of emodin in experimental animals. Two-Year Chronic Study: The two-year study will evaluate the chronic toxicity and carcinogenicity of emodin administered to male and female F344 rats and. B6C3F1 mice. All moribund animals, as well as those that survive to the end of the study, will receive a complete gross examination. All grossly visible lesions and all tissues with non-neoplastic or neoplastic lesions will receive a complete histopathologic examination.

这项调查的具体目标是评估 大黄素的慢性前期和慢性毒性及致癌性 Fischer-344大鼠和B6C3F1小鼠。以下研究将是 已执行： 14天重复给药的慢性前期毒性研究：本研究 将表征与膳食暴露相关的毒性 大黄素包括靶器官的鉴别、测定 无影响水平(如果可行)、敏感度差异 性别和物种之间的差异，以及剂量反应的斜率 曲线。 为期13周(90天)的亚慢性研究：除了获得 在14天重复剂量下描述的毒理学数据 毒性研究，这项研究也将有助于确定剂量 在为期两年的慢性毒性研究中，针对每个菌株和物种。 这项检查包括尸检和组织病理学。 评估、制备未染色的血液涂片(供 NTP在微核试验中的应用)和血液测定 大黄素在实验动物体内的浓度。 为期两年的慢性研究：这项为期两年的研究将评估 大黄素对男性的慢性毒性和致癌性 雌性F344大鼠和。B6C3F1小鼠。所有垂死的动物，如 以及那些活到研究结束的人，将收到 全面的肉眼检查。所有肉眼可见的损伤和所有 非肿瘤性或肿瘤性损害的组织将接受 完整的组织病理学检查。