PRECHRONIC & CHRONIC TOXICITY OF EMODIN

本次调查的具体目标是评估 大黄素慢性前期和慢性毒性及致癌性 Fischer-344 大鼠和 B6C3F1 小鼠。 将进行以下研究 执行： 十四天重复剂量慢性前期毒性研究：本研究 将表征与饮食暴露相关的毒性 大黄素包括靶器官的鉴定、测定 无影响水平（如果可行），敏感性差异 性别和物种之间的差异，以及剂量反应的斜率 曲线。 十三周（90 天）亚慢性研究：除了获得 毒理学数据如 14 天重复剂量所述 毒性研究，这项研究还将有助于确定剂量 对于为期 2 年的慢性毒性研究中的每个菌株和物种。 这项调查包括尸检和组织病理学 评估、准备未染色的血涂片（供 微核测定中的 NTP）和血液测定 实验动物中大黄素的浓度。 为期两年的慢性研究：为期两年的研究将评估 大黄素对男性的慢性毒性和致癌性 和雌性F344大鼠和。 B6C3F1 小鼠。 所有垂死的动物，如 以及那些存活到研究结束的人，将获得 完整的粗略检查。 所有肉眼可见的病变和所有 具有非肿瘤性或肿瘤性病变的组织将接受 完成组织病理学检查。

The specific objectives of this investigation is to evaluate the prechronic and chronic toxicity and carcinogenicity of emodin in Fischer-344 rats and B6C3F1 mice. The following studies will be performed: Fourteen-Day Repeated-Dose Prechronic Toxicity Study: This study will characterize the toxicity associated with dietary exposure to emodin including the identification of target organs, determination of a no-effect level (if feasible), differences in sensitivity between sexes and species, and the slope of the dose-response curve. Thirteen-Week (90-Day) Subchronic Study: In addition to obtaining toxicological data as described under the 14-day repeated-dose toxicity study, this study will also help to determine the doses for each strain and species in the chronic 2-year toxicity study. This investigation includes necropsy and histopathologic evaluations, preparation of unstained blood smears (for use by the NTP in micronuleic determinations) and determination of blood concentrations of emodin in experimental animals. Two-Year Chronic Study: The two-year study will evaluate the chronic toxicity and carcinogenicity of emodin administered to male and female F344 rats and. B6C3F1 mice. All moribund animals, as well as those that survive to the end of the study, will receive a complete gross examination. All grossly visible lesions and all tissues with non-neoplastic or neoplastic lesions will receive a complete histopathologic examination.