SOLID STATE MAGNETIC RESONANCE OF BONE MINERAL

这项研究的长期目标是定义固态 成熟期不同阶段的骨矿化学 成人成熟骨中最早的沉积过程 生物体，以及与愈合相关的各种重塑步骤 和骨骼相关的疾病。尽管进行了多年的研究，但有一种 正常人在骨矿化学知识上的明显差距 在骨折愈合期间和疾病中的发育中的生物体 涉及到骨矿物质。骨质疏松症就是这种疾病的一个例子。 它已经成为公共卫生的一个重要问题，以及 尤其是妇女的健康。 具体地说，我们将表征并尝试识别该化学物质 最早的新矿物中涉及的磷酸钙的形态 在发育中的生物体的沉积过程中和在兔子的钻头中 缺陷愈合的孔洞模型。我们所获得的知识有望 加深我们对基本生物过程的了解 涉及骨的沉积和吸收，以及我们对 与骨骼相关的疾病。 为了实现这些目标，我们将开发和应用新型固体 态(31)P交叉极化磁共振波谱和 骨的体外和体内评价的成像技术 矿物化学。固态磁共振成像和光谱分析的应用 在很大程度上未被开发的骨骼具有巨大的增长潜力 关于骨形成、吸收、生长和愈合的知识，以及 在体内非侵入性地遵循这些过程。方法和方法 我们开发的设备也将应用于，并产生类似 在人造材料和装置研究方面的优势，包括 硅胶植入物，以及聚合物、复合材料和陶瓷假体。 该项目的具体目标包括：1)提炼和验证 固态磁共振成像和光谱技术实现的程度 对骨骼的持续研究所需的定量准确性 矿物开发；2)提高固态交联剂的配伍性 与活体受试者的偏振成像和光谱； 兔胫骨钻头早期矿物质的特征及其成熟度 活体(31)P固体交叉极化成像的空穴模型 局域光谱和体外高场差分交叉 偏振光谱。

The long-term goal of this research is to define the solid state chemistry of bone mineral at various stages of maturation from the earliest deposition-processes through fully mature bone in adult organisms, and in the various remodeling steps associated with healing and bone-related diseases. Despite many years of research, there is a glaring gap in our knowledge of bone mineral chemistry in normal developing organisms, during the healing of fractures, and in diseases involving bone mineral. Osteoporosis is an example of one such disease which has assumed major proportions as an issue in public health, and women's health in particular. Specifically, we will characterize and attempt to identify the chemical forms of calcium phosphate involved in the earliest new mineral laid down in deposition processes in developing organisms and in a rabbit drill hole model of defect healing. The knowledge we gain is expected to enhance our understanding of the fundamental biological processes involving deposition and resorption of bone, as well as our understanding of bone-related diseases. In order to achieve these goals, we will develop and apply novel solid state (31)P cross polarization magnetic resonance spectroscopy and imaging techniques for the in vitro and in vivo assessment of bone mineral chemistry. The use of solid state MR imaging and spectroscopy of bone, largely unexplored, holds enormous potential for increasing knowledge of bone formation, resorption, growth, and healing, and for following these processes in the body noninvasively. The methods and apparatus we develop will have application also to, and yield similar advantages in, studies of artificial materials and devices, including silicone implants, and polymeric, composite and ceramic prostheses. The specific aims of the project include 1) refining and validating these solid state MR imaging and spectroscopic techniques to achieve the degree of quantitative accuracy necessary for continuing research on bone mineral development; 2) improving the compatibility of solid state cross polarization imaging and spectroscopy with living subjects; and 3) characterizing early mineral and its maturation in a rabbit tibia drill hole model by in vivo (31)P solid state cross polarization imaging and localized spectroscopy, and ex vivo high field differential cross polarization spectroscopy.