GALECTIN-2, AN ANIMAL LECTIN, IN ALLERGIC INFLAMMATION
Galectin-2(一种动物凝集素)在过敏性炎症中的作用
基本信息
- 批准号:2004665
- 负责人:
- 金额:$ 21.05万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-04-01 至 2000-03-31
- 项目状态:已结题
- 来源:
- 关键词:antibody receptor chemical structure function clinical research disease /disorder model epithelium human subject hypersensitivity inflammation laboratory mouse lectin leukocyte activation /transformation macrophage mast cell model design /development molecular pathology protein sequence tissue /cell culture
项目摘要
This research program deals with a protein that we have identified through
its IgE-binding activity and extensively studied. This protein is now
known as galectin-3, a member of a large family of beta-galactoside-binding
animal lectins. We have accumulated a great deal of information on various
aspects of galectin-3 which has allowed us to hypothesize that galectin-3
is a broad-spectrum amplifier of inflammatory response. We propose to
continue to study the role of galectin-3 in inflammation by focusing on the
following three specific aims.
1. Establishment of the function of galectin-3 inflammatory cells. We
have shown various activities of galectin-3 by using exogenously added
protein. THe role of galectin-3 in inflammation will be more firmly
established by demonstrating that the endogenous lectin can participate in
these activities. CO-culture systems in which galectin-3 secreted from
macrophages and epithelial cells activates mast cells will be studied as
examples. We will first establish conditions in which galectin-3 is
released by inflammatory cells. The activity of galectin-3 i
potentialteing the activation of inflammatory cells induced by other
stimuli will also be investigated. Cells from galectin-3 mice will be used
extensively for these analyses.
2. Development of animal models for establishing the role of galectin-3
inflammation. MOuse models of airway inflammation and cutaneous
inflammation will be used to analyze the expression and secretion of
galectin-3 in inflamatory responses. Galectin-3-deficient mice will be
used to evaluate the role of galaectin-3 in airway and cutaneous
inflammation.
3. Elucidation of the molecular basis for galectinn-3's activation of
inflammatory cells. The realtionship between galectin-3's activation of
mast cells and its recognition of Fc (epsilon) RI will be investigated.
Molecular requirements for galectin-3's abilty to activates leukocytes will
be delineated by focusing on galectin-3's self association property.
Covalent galectin-3 dimers will be generated and compared with the
mononomeric protein for various activities. AMino acid sequence in the
amino-terminal region that is responsible for galectin-3's self association
will be identified.
该研究计划涉及我们通过以下方式鉴定的蛋白质
其 IgE 结合活性并得到广泛研究。 这种蛋白质现在
称为 galectin-3,β-半乳糖苷结合大家族的成员
动物凝集素。 我们积累了大量的各种信息
半乳糖凝集素 3 的各个方面,这使我们能够假设半乳糖凝集素 3
是一种广谱炎症反应放大器。 我们建议
继续研究半乳糖凝集素 3 在炎症中的作用
遵循三个具体目标。
1. galectin-3炎症细胞功能的建立。 我们
通过使用外源添加,显示了半乳糖凝集素 3 的各种活性
蛋白质。 Galectin-3在炎症中的作用将更加牢固
通过证明内源性凝集素可以参与
这些活动。 半乳糖凝集素 3 分泌的共培养系统
巨噬细胞和上皮细胞激活肥大细胞将被研究为
例子。 我们首先建立半乳糖凝集素3存在的条件
由炎症细胞释放。 Galectin-3 i 的活性
潜在地激活其他物质诱导的炎症细胞
刺激因素也将受到调查。将使用来自 galectin-3 小鼠的细胞
广泛用于这些分析。
2. 建立半乳糖凝集素3作用的动物模型
炎。 气道炎症和皮肤炎症小鼠模型
炎症将用于分析表达和分泌
半乳糖凝集素 3 在炎症反应中的作用。 半乳糖凝集素 3 缺陷小鼠将
用于评估 galaectin-3 在气道和皮肤中的作用
炎。
3. 阐明galectinn-3激活的分子基础
炎症细胞。 galectin-3的激活关系
将研究肥大细胞及其对 Fc (epsilon) RI 的识别。
半乳糖凝集素 3 激活白细胞能力的分子要求将
通过关注 galectin-3 的自缔合特性来描述。
将生成共价半乳糖凝集素 3 二聚体并与
具有多种活性的单体蛋白质。 中的氨基酸序列
负责 galectin-3 自缔合的氨基末端区域
将被识别。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(1)
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{{ truncateString('FU-TONG LIU', 18)}}的其他基金
DEVELOPMENT OF NONHUMAN PRIMATE MODELS OF ATOPIC DERMATITIS
特应性皮炎非人灵长类动物模型的开发
- 批准号:
8357349 - 财政年份:2011
- 资助金额:
$ 21.05万 - 项目类别:
Galectin-3 in regulation of allergic skin inflammation
Galectin-3 调节过敏性皮肤炎症
- 批准号:
7785910 - 财政年份:2010
- 资助金额:
$ 21.05万 - 项目类别:
DEVELOPMENT OF NONHUMAN PRIMATE MODELS OF ATOPIC DERMATITIS
特应性皮炎非人灵长类动物模型的开发
- 批准号:
8172632 - 财政年份:2010
- 资助金额:
$ 21.05万 - 项目类别:
Galectin-3 in regulation of allergic skin inflammation
Galectin-3 调节过敏性皮肤炎症
- 批准号:
8401847 - 财政年份:2010
- 资助金额:
$ 21.05万 - 项目类别:
Galectin-3 in regulation of allergic skin inflammation
Galectin-3 调节过敏性皮肤炎症
- 批准号:
8013555 - 财政年份:2010
- 资助金额:
$ 21.05万 - 项目类别:
Galectin-3 in regulation of allergic skin inflammation
Galectin-3 调节过敏性皮肤炎症
- 批准号:
8206814 - 财政年份:2010
- 资助金额:
$ 21.05万 - 项目类别:
Regulation of Skin Wound Epithelialization by Galectin-3
Galectin-3 调节皮肤伤口上皮化
- 批准号:
7470573 - 财政年份:2007
- 资助金额:
$ 21.05万 - 项目类别:
Regulation of Skin Wound Epithelialization by Galectin-3
Galectin-3 调节皮肤伤口上皮化
- 批准号:
7315928 - 财政年份:2007
- 资助金额:
$ 21.05万 - 项目类别:
Validation of Galectin-3 as a Target in Cancer Therapy
Galectin-3 作为癌症治疗靶点的验证
- 批准号:
6640748 - 财政年份:2002
- 资助金额:
$ 21.05万 - 项目类别:
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