DEVELOPMENT OF NONHUMAN PRIMATE MODELS OF ATOPIC DERMATITIS

特应性皮炎非人灵长类动物模型的开发

• 批准号: 8172632
• 负责人: FU-TONG LIU
• 金额: $ 7.6万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172632
• 关键词: Allergens; Atopic Dermatitis; Bacteria; Biopsy; Characteristics; Clinic; Computer Retrieval of Information on Scientific Projects Database; Dermatitis; Development; Exanthema; Funding; Grant; House Dust Mite Allergens; IgE; Institution; Macaca mulatta; Modeling; Monitor; Monkeys; Pilot Projects; Punch Biopsy; Recording of previous events; Research; Research Personnel; Resources; Serum; Skin; Source; United States National Institutes of Health; atopy; nonhuman primate; skin lesion

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this pilot project we propose to study two rhesus monkey models of atopic dermatitis. One is a spontaneously occurring model. Monkeys admitted to the clinic for a rash will be examined for the presence of characteristic pruritic eczematous skin lesions, history of atopy, typical distribution of the rash, dry skin, and high bacteria colonization. A skin biopsy of the lesional skin will be obtained for histochemical analysis. Sera will be obtained for the total IgE levels. The other is an allergen-induced model. We will induce dermatitis in monkeys by three one-week periods of epicutaneous application of house-dust mite allergens under occlusion, at two-week intervals. 6-mm skin punch biopsies will be performed before and after the 3rd epicutaneous application. Sera will be collected before and after each epicutaneous application. Biopsies and sera will be analyzed to monitor the development of atopic dermatitis.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在这个试点项目中，我们建议研究两种恒河猴特应性皮炎模型。一种是自发发生的模型。因皮疹入院的猴子将接受检查，以确定是否存在典型的瘙痒性湿疹皮损、特应性病史、皮疹的典型分布、皮肤干燥和高度细菌定植。将获得皮损皮肤的皮肤活检以进行组织化学分析。将获得总IgE水平的血清。另一种是过敏原诱导的模型。我们将在封闭状态下，每隔两周，通过三次为期一周的皮肤表面应用屋尘螨变应原，诱导猴子皮炎。在第3次外用之前和之后，将进行6 mm皮肤穿孔活检。每次皮肤外用前后都会采集血清。将对活检和血清进行分析，以监测特应性皮炎的发展。