DEVELOPMENT OF NONHUMAN PRIMATE MODELS OF ATOPIC DERMATITIS

特应性皮炎非人灵长类动物模型的开发

• 批准号: 8172632
• 负责人: FU-TONG LIU
• 金额: $ 7.6万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172632
• 关键词: Allergens; Atopic Dermatitis; Bacteria; Biopsy; Characteristics; Clinic; Computer Retrieval of Information on Scientific Projects Database; Dermatitis; Development; Exanthema; Funding; Grant; House Dust Mite Allergens; IgE; Institution; Macaca mulatta; Modeling; Monitor; Monkeys; Pilot Projects; Punch Biopsy; Recording of previous events; Research; Research Personnel; Resources; Serum; Skin; Source; United States National Institutes of Health; atopy; nonhuman primate; skin lesion

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this pilot project we propose to study two rhesus monkey models of atopic dermatitis. One is a spontaneously occurring model. Monkeys admitted to the clinic for a rash will be examined for the presence of characteristic pruritic eczematous skin lesions, history of atopy, typical distribution of the rash, dry skin, and high bacteria colonization. A skin biopsy of the lesional skin will be obtained for histochemical analysis. Sera will be obtained for the total IgE levels. The other is an allergen-induced model. We will induce dermatitis in monkeys by three one-week periods of epicutaneous application of house-dust mite allergens under occlusion, at two-week intervals. 6-mm skin punch biopsies will be performed before and after the 3rd epicutaneous application. Sera will be collected before and after each epicutaneous application. Biopsies and sera will be analyzed to monitor the development of atopic dermatitis.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在这个试点项目中，我们建议研究两个特应性皮炎的恒河猴模型。一个是自发发生的模型。 将检查诊所接受皮疹的猴子是否存在特征性的核炎性皮肤病变，特应性史，皮疹，干燥皮肤干燥和高细菌定殖的典型分布。 将获得病变皮肤的皮肤活检，以进行组织化学分析。将获得总IgE水平的血清。另一个是过敏原诱导的模型。我们将以两周的间隔在闭塞下诱导猴子的三个星期施用猴子的猴子皮炎。 在第三次表皮施用之前和之后，将进行6毫米的皮肤拳活检。 每次涂抹之前和之后，将收集血清。 活检和血清将进行分析，以监测特应性皮炎的发育。