DEVELOPMENT OF NONHUMAN PRIMATE MODELS OF ATOPIC DERMATITIS

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 表示子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在这个试点项目中，我们建议研究两个恒河猴模型的特应性皮炎。一个是自发发生的模型。 将检查因皮疹进入诊所的猴是否存在特征性湿疹性皮肤病变、特应性病史、皮疹的典型分布、皮肤干燥和高细菌定植。 将获得病变皮肤的皮肤活检用于组织化学分析。将获得血清以测定总IgE水平。另一种是过敏原诱导模型。我们将通过在封闭条件下，以两周为间隔，对猴进行三次为期一周的屋尘螨过敏原表皮应用，诱导猴皮炎。 在第3次表皮贴敷前后进行6 mm皮肤穿刺活检。 将在每次表皮贴敷前后采集血清。 将对活检和血清进行分析，以监测特应性皮炎的发展。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In this pilot project we propose to study two rhesus monkey models of atopic dermatitis. One is a spontaneously occurring model. Monkeys admitted to the clinic for a rash will be examined for the presence of characteristic pruritic eczematous skin lesions, history of atopy, typical distribution of the rash, dry skin, and high bacteria colonization. A skin biopsy of the lesional skin will be obtained for histochemical analysis. Sera will be obtained for the total IgE levels. The other is an allergen-induced model. We will induce dermatitis in monkeys by three one-week periods of epicutaneous application of house-dust mite allergens under occlusion, at two-week intervals. 6-mm skin punch biopsies will be performed before and after the 3rd epicutaneous application. Sera will be collected before and after each epicutaneous application. Biopsies and sera will be analyzed to monitor the development of atopic dermatitis.