DEVELOPMENT OF NONHUMAN PRIMATE MODELS OF ATOPIC DERMATITIS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在这个试点项目中，我们建议研究两种恒河猴特应性皮炎模型。一种是自发发生的模型。因皮疹入院的猴子将接受检查，以确定是否存在典型的瘙痒性湿疹皮损、特应性病史、皮疹的典型分布、皮肤干燥和高度细菌定植。将获得皮损皮肤的皮肤活检以进行组织化学分析。将获得总IgE水平的血清。另一种是过敏原诱导的模型。我们将在封闭状态下，每隔两周，通过三次为期一周的皮肤表面应用屋尘螨变应原，诱导猴子皮炎。在第3次外用之前和之后，将进行6 mm皮肤穿孔活检。每次皮肤外用前后都会采集血清。将对活检和血清进行分析，以监测特应性皮炎的发展。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In this pilot project we propose to study two rhesus monkey models of atopic dermatitis. One is a spontaneously occurring model. Monkeys admitted to the clinic for a rash will be examined for the presence of characteristic pruritic eczematous skin lesions, history of atopy, typical distribution of the rash, dry skin, and high bacteria colonization. A skin biopsy of the lesional skin will be obtained for histochemical analysis. Sera will be obtained for the total IgE levels. The other is an allergen-induced model. We will induce dermatitis in monkeys by three one-week periods of epicutaneous application of house-dust mite allergens under occlusion, at two-week intervals. 6-mm skin punch biopsies will be performed before and after the 3rd epicutaneous application. Sera will be collected before and after each epicutaneous application. Biopsies and sera will be analyzed to monitor the development of atopic dermatitis.