BIOPRINTER FOR MYCOBACTERIUM DIAGNOSTIC ARRAYS

用于分枝杆菌诊断阵列的生物打印机

• 批准号: 2005337
• 负责人: Donald Hayes
• 金额: $ 9.99万
• 依托单位: MICROFAB TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2005337
• 关键词: Mycobacterium tuberculosis; bacterial genetics; biomedical equipment development; biotechnology; clinical biomedical equipment; communicable disease diagnosis; computer graphics /printing; computer system design /evaluation; diagnosis design /evaluation; fluid flow; method development; nucleic acid hybridization; nucleic acid probes; oligonucleotides

We will build a multichannel "bioprinter" for high-speed printing of DNA probe microspots. This technology provides an essential foundation for the coming BioChip revolution in diagnostic medicine. Virtually all of the high-density, biochip-style devices in development will demand fast, practical, and accurate methods of dispensing micronscale spots of biological reagents. MicroFab's ink-jet technology can extend biological fluid dispensing capabilities by 1000-fold in miniaturization and 100-fold in speed, making biochip production a practical reality. We will design our multichannel printer for the specific task of producing a diagnostic system for M. tuberculosis (Mtb). The long-range goal is to produce a robust, hand-held diagnostic kit that can give rapid ( l hr) identification of drug-resistant strains of the mycobacterium from clinical specimens. By the use of printed high-density DNA capture probe arrays, as many as 400 allelic variants can be probed for in one cm2. Thus, genomic screening for hundreds of Mtb variants can be envisioned within a massively-parallel, one-step kit format. In Phase I we will test multichannel printhead designs, using clinically- important Mtb DNA probes to assess the fidelity of final probe-target hybridization. Phase II should conclude with prototype arrays manufactured for experimental testing on clinical specimens. PROPOSED COMMERCIAL APPLICATION: M. tuberculosis is the number one killer among infectious diseases. Technology for producing fast diagnostic systems will be extremely valuable. More generally, a BioPrinter capable of producing immuno- and DNA-probe arrays at microscopic scale and high volume is needed throughout biomedicine and biotechnology .The system we will develop could easily become the preferred technology for all diagnostic array production world-wide.

我们将建造一台用于高速打印DNA的多通道“生物打印机” 探测微斑点。这项技术为 即将到来的诊断医学生物芯片革命。几乎所有的 正在开发的高密度生物芯片式设备将需要快速、 一种实用、准确的微米级斑点分配方法 生物试剂。MicroFab的喷墨技术可以扩展生物 液体分配能力在小型化中提高1000倍，在100倍中提高 在速度上，使生物芯片生产成为现实。 我们将为生产的特定任务设计我们的多通道打印机 结核分枝杆菌诊断系统(MTB)。长期目标是 生产一种坚固耐用的手持诊断试剂盒，能够提供快速诊断(L人力资源) 临床分离的分支杆菌耐药株的鉴定 标本。通过使用印刷的高密度DNA捕获探针阵列， 在一平方厘米内可以探测到多达400个等位基因变异。因此，基因组学 可以设想在一年内筛查数百种结核分枝杆菌变种 大规模并行、一步式套件格式。 在第一阶段，我们将测试多通道打印头设计，在临床上使用- 重要的结核分枝杆菌DNA探针用于评估最终探针-靶的保真度 杂交。第二阶段应以制造原型阵列结束 在临床标本上进行实验测试。 建议商业应用：结核分枝杆菌是头号杀手 在传染病中。用于生产快速诊断系统的技术 将是非常有价值的。更广泛地说，生物打印机能够 生产微观规模和大批量的免疫和DNA探针阵列 在整个生物医学和生物技术中都是需要的。我们将 开发可以很容易地成为所有诊断的首选技术 世界各地的阵列生产。