COMPUTER AIDED TWO-DIMENSIONAL ELECTROPHORETIC GEL ANALYSIS

GELLAB-II软件系统是一个探索性的数据分析系统 用于分析2D电泳蛋白质凝胶图像集。 它 结合了用于图像采集的复杂子系统， 处理、数据库操作、图形和统计分析。 它已被应用于各种实验系统，其中 一种或多种蛋白质的定量和定性变化， 数百或数千个未改变的蛋白质是基本的分析 问题. 跟踪使用这些方法检测到的更改也是 这是该系统的一个主要特征。 复合凝胶数据库可以是 在不同的探索性数据分析条件下"查看"， 从"切片"中阐明的统计差异和微妙模式 一个有效的3D数据库 结果可以以多种形式呈现 表格、绘图或衍生图像以及在工作站上的大面积 网络. GELLAB-II研究导致了技术转让 CRADA与CSPI/Scalytics Inc.对于市售系统 GELLAB-II+使这项技术更容易用于癌症 研究人员在廉价的微软Windows PC（1996年发布）。 这种商业化的结果是更广泛地使用和更好地支持 GELLAB-II技术比我们可以提供。 GELLAB-II应用程序 期间包括：正在进行的核基质蛋白变化的研究， 前列腺癌，以帮助筛查和分期的男性前列腺 癌症（J.霍普金斯）;和Rett综合征（CDC）罗宾逊MK等。 电泳1995; 16：1176 - 83. 在前列腺癌研究中，我们 正在寻找缺失的蛋白质以及 蛋白质组模式的变化， 前列腺疾病男性患者的实验条件、分期和筛查 癌 我们也在对核基质进行类似的研究 肾细胞癌的蛋白质模式 霍普金斯集团 前列腺分子工作已经确定了几个蛋白质点， 序列分析，并正在完成研究，以确认这些 序列的

The GELLAB-II software system is an exploratory data analysis system for the analysis of sets of 2D electrophoretic protein gel images. It incorporates sophisticated subsystems for image acquisition, processing, database manipulation, graphics and statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes in one or more proteins among hundreds or thousands of unaltered proteins is the basic analytic problem. Keeping track of changes detected using these methods is also a major attribute of the system. A composite gel database may be "viewed" under different exploratory data analysis conditions and statistical differences and subtle patterns elucidated from "slices" of an effectively 3D database. Results can be presented in a variety of tables, plots or derived images and on workstations over wide area networks. The GELLAB-II research has resulted in a technology transfer CRADA with CSPI/Scanalytics Inc. for a commercially available system GELLAB-II+ making this technology easily available to cancer researchers on inexpensive Microsoft Windows PCs (released in 1996). Such commercialization results in wider use and better support of the GELLAB-II technology than we can provide. GELLAB-II applications this period include: ongoing studies of nuclear matrix protein changes in prostate cancer to aid screening and staging of men with prostate cancer (J.Hopkins); and Rett syndrome (CDC) Robinson MK etal. Electrophoresis 1995;16:1176-83. In the prostate cancer studies, we are looking for both missing proteins as well as subtle quantitative changes in patterns on sets of proteins which correlates with experimental conditions and staging and screening of men with prostate cancer. We are also doing a similar investigation of nuclear matrix protein patterns from renal cell carcinoma. The Hopkins group's prostate molecular work has identified several of the protein spots by sequence analysis and are completing studies to confirm these sequences.