COMPUTER AIDED TWO-DIMENSIONAL ELECTROPHORETIC GEL ANALYSIS

计算机辅助二维电泳凝胶分析

• 批准号: 2463736
• 负责人: P LEMKIN
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2463736
• 关键词: Rett syndrome; computer assisted diagnosis; computer data analysis; computer network; computer program /software; computer system design /evaluation; gel electrophoresis; human data; human genetic material tag; image processing; information systems; mass screening; neoplasm /cancer classification /staging; nuclear matrix; prostate neoplasms; protein sequence; renal cell carcinoma

The GELLAB-II software system is an exploratory data analysis system for the analysis of sets of 2D electrophoretic protein gel images. It incorporates sophisticated subsystems for image acquisition, processing, database manipulation, graphics and statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes in one or more proteins among hundreds or thousands of unaltered proteins is the basic analytic problem. Keeping track of changes detected using these methods is also a major attribute of the system. A composite gel database may be "viewed" under different exploratory data analysis conditions and statistical differences and subtle patterns elucidated from "slices" of an effectively 3D database. Results can be presented in a variety of tables, plots or derived images and on workstations over wide area networks. The GELLAB-II research has resulted in a technology transfer CRADA with CSPI/Scanalytics Inc. for a commercially available system GELLAB-II+ making this technology easily available to cancer researchers on inexpensive Microsoft Windows PCs (released in 1996). Such commercialization results in wider use and better support of the GELLAB-II technology than we can provide. GELLAB-II applications this period include: ongoing studies of nuclear matrix protein changes in prostate cancer to aid screening and staging of men with prostate cancer (J.Hopkins); and Rett syndrome (CDC) Robinson MK etal. Electrophoresis 1995;16:1176-83. In the prostate cancer studies, we are looking for both missing proteins as well as subtle quantitative changes in patterns on sets of proteins which correlates with experimental conditions and staging and screening of men with prostate cancer. We are also doing a similar investigation of nuclear matrix protein patterns from renal cell carcinoma. The Hopkins group's prostate molecular work has identified several of the protein spots by sequence analysis and are completing studies to confirm these sequences.

GELLAB-II软件系统是一个探索性的数据分析系统 用于分析多组2D电泳蛋白质凝胶图像。它 集成了用于图像采集的复杂子系统， 处理、数据库操作、绘图和统计分析。 它已被应用于各种实验系统，在这些系统中 一种或多种蛋白质的数量和质量变化 成百上千的未改变的蛋白质是基本的分析 有问题。跟踪使用这些方法检测到的变化也是 这是该系统的一个主要属性。复合凝胶数据库可以是 在不同的探索性数据分析条件下和 从《切片》看统计差异和微妙模式 一个有效的3D数据库。结果可以以不同的形式呈现 大范围的表格、绘图或派生图像以及工作站上的 网络。GELLAB-II的研究已经导致了技术转移 CRADA与CSPI/Scanalytics Inc.合作开发商用系统 GELLAB-II+让这项技术更容易为癌症所用 研究人员在廉价的Microsoft Windows PC上(1996年发布)。 这种商业化导致了更广泛的使用和更好的支持 GELLAB-II技术是我们所能提供的。GELLAB-II应用本 期间包括：正在进行的关于核基质蛋白变化的研究 前列腺癌有助于前列腺癌患者的筛查和分期 癌症(J.Hopkins)和Rett综合征(CDC)Robinson MK等。 电泳版1995；16：1176-83。在前列腺癌研究中，我们 正在寻找缺失的蛋白质以及微妙的定量 与之相关的一组蛋白质模式的变化 男性前列腺癌的实验条件、分期和筛查 癌症。我们也在对核基质进行类似的研究 肾细胞癌的蛋白质图谱。霍普金斯研究小组的 前列腺分子研究已经确定了几个蛋白点，通过 序列分析，并正在完成研究，以证实这些 序列。