COMPUTER AIDED TWO-DIMENSIONAL ELECTROPHORETIC GEL ANALYSIS

计算机辅助二维电泳凝胶分析

• 批准号: 3808527
• 负责人: P LEMKIN
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3808527
• 关键词: axon; cadmium; cell differentiation; computer graphics /printing; computer system design /evaluation; cytotoxicity; gel electrophoresis; gene expression; genetic manipulation; image processing; molecular dynamics; molecular oncology; nucleic acid sequence; prostate neoplasms; protein biosynthesis; protein sequence; tissue /cell culture

Analytic methods are continuing to be developed and applied using the GELLAB-II software system - an exploratory data analysis system for the analysis of sets of 2D electrophoretic gel images. It incorporates sophisticated subsystems for image acquisition, processing, database manipulation, graphics and statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes in one or more proteins among hundreds or thousands of unaltered proteins is the basic analytic problem. Keeping track of changes detected using these methods is also a major attribute of the system. A composite gel database may be "viewed" under different exploratory data analysis conditions and statistical differences and subtle patterns elucidated from "slices" of an effectively 3D database. Results can be presented in a variety of tables, plots or derived images. It runs on UNIX workstations and the CONVEX supercomputer using X-windows for portable interactive graphics. Recently, remote collaborative multimedia image-conferencing has been added to GELLAB-II with possible use in other fields of research. Substantive applications include: assess stability of long term tumor cell lines (Alley); 2D DNA gels for identifying differentially expressed genes (Rogan and Strathern); cadmium toxicity in urine (Myrick); analyzing sets of lymphocyte gels and long term cell lines from human hematological malignancies to identify potential marker proteins for possible microsequencing (Lester); cerebral endothelial cell morphology control (Bauer and Amberger); transcription control in prostrate cell lines (Getzenberg). GELLAB-II has been exported to the U. of Zurich (Sonderegger and Hale), CDC/Atlanta (Myrick), U. Norway (Krekling) . Additional changes to help continued collaboration and exporting of enhanced versions of GELLAB has been actively pursued.

分析方法正在继续开发和应用 GELLAB-II软件系统-一种用于 2D电泳凝胶图像组的分析。 而纳入 用于图像采集、处理、数据库 操作、图形和统计分析。 它已被应用到 各种实验系统，其中定量和定性 一种或多种蛋白质在数百或数千种未改变的蛋白质中发生变化， 蛋白质是基本的分析问题。 跟踪更改 使用这些方法检测也是系统的主要属性。 一 复合凝胶数据库可以在不同的探索性数据下"查看" 分析条件和统计差异以及细微模式 从有效的3D数据库的"切片"中阐明。 结果可 以各种表格、图或衍生图像呈现。 它运行在 UNIX工作站和CONVEX超级计算机使用X-windows， 便携式交互图形。 最近，远程协作多媒体 GELLAB-II增加了图像会议功能，可能用于其他 研究领域。 实质应用包括：评估长期肿瘤的稳定性 细胞系（Alley）;用于鉴定差异表达的2D DNA凝胶 尿镉毒性（Myrick）; 分析来自人类的淋巴细胞凝胶和长期细胞系 血液恶性肿瘤，以确定潜在的标志物蛋白， 可能的微测序（Lester）;脑内皮细胞形态 控制（Bauer和Amberger）;前列腺细胞中的转录控制 线（Getzenberg）。 GELLAB-II已出口美国。关于苏黎世 （Sonderegger和黑尔），CDC/亚特兰大（Myrick），美国。 挪威（Krekling）。 其他更改有助于继续协作和导出 GELLAB的增强版本已经被积极地追求。