COMPUTER AIDED TWO-DIMENSIONAL ELECTROPHORETIC GEL ANALYSIS

分析方法正在继续开发和应用，使用的是 GELLAB-II软件系统--探索性数据分析系统 对多组二维电泳胶图像进行分析。它包含了 用于图像采集、处理、数据库的复杂子系统 操纵、图表和统计分析。它已被应用于 各种实验系统，其中定量和定性 在成百上千种未改变的蛋白质中，一种或多种蛋白质的变化 蛋白质是基本的分析问题。跟踪更改 使用这些方法进行检测也是该系统的一个主要属性。一个 复合凝胶数据库可以在不同的勘探数据下进行查看 分析条件和统计差异以及微妙的模式 从一个有效的3D数据库的“切片”中阐明。结果可以是 以各种表格、曲线图或衍生图像的形式呈现。它继续运行 Unix工作站和使用X-WINDOWS的凸形超级计算机 便携交互式图形。最近，远程协作多媒体 图像会议已添加到GELLAB-II中，可能用于其他 研究领域。 实质性应用包括：评估长期肿瘤的稳定性 细胞系(小巷)：用于鉴定差异表达的2D DNA凝胶 基因(Rogan和Strathern)；尿镉毒性(Myrick)； 分析人类多组淋巴细胞凝胶和长期细胞系 恶性血液病识别潜在标志物蛋白的研究 可能的微测序(莱斯特)；脑内皮细胞形态 控制(Bauer和Amberger)；胰岛细胞的转录控制 Lines(Getzenberg)。GELLAB-II已出口到美国苏黎世 (Sonderegger和Hale)，CDC/亚特兰大(Myrick)，英国挪威(Krekling)。 其他更改以帮助继续协作和导出 GELLAB的增强版本一直在积极寻求。

Analytic methods are continuing to be developed and applied using the GELLAB-II software system - an exploratory data analysis system for the analysis of sets of 2D electrophoretic gel images. It incorporates sophisticated subsystems for image acquisition, processing, database manipulation, graphics and statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes in one or more proteins among hundreds or thousands of unaltered proteins is the basic analytic problem. Keeping track of changes detected using these methods is also a major attribute of the system. A composite gel database may be "viewed" under different exploratory data analysis conditions and statistical differences and subtle patterns elucidated from "slices" of an effectively 3D database. Results can be presented in a variety of tables, plots or derived images. It runs on UNIX workstations and the CONVEX supercomputer using X-windows for portable interactive graphics. Recently, remote collaborative multimedia image-conferencing has been added to GELLAB-II with possible use in other fields of research. Substantive applications include: assess stability of long term tumor cell lines (Alley); 2D DNA gels for identifying differentially expressed genes (Rogan and Strathern); cadmium toxicity in urine (Myrick); analyzing sets of lymphocyte gels and long term cell lines from human hematological malignancies to identify potential marker proteins for possible microsequencing (Lester); cerebral endothelial cell morphology control (Bauer and Amberger); transcription control in prostrate cell lines (Getzenberg). GELLAB-II has been exported to the U. of Zurich (Sonderegger and Hale), CDC/Atlanta (Myrick), U. Norway (Krekling) . Additional changes to help continued collaboration and exporting of enhanced versions of GELLAB has been actively pursued.