COMPUTER AIDED TWO-DIMENSIONAL ELECTROPHORETIC GEL ANALYSIS

计算机辅助二维电泳凝胶分析

• 批准号: 3752038
• 负责人: P LEMKIN
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752038
• 关键词: Rett syndrome; cadmium; cerebrospinal fluid; computer human interaction; computer system design /evaluation; dioxins; fetal alcohol syndrome; gel electrophoresis; gene expression; image processing; information systems; mercury poisoning; metal poisoning; plasma; protein purification; radiation hazard; serum; toxicology; urine

Analytic methods are continuing to be developed and applied using the GELLAB-II software system - an exploratory data analysis system for the analysis of sets of 2D electrophoretic gel images. It incorporates sophisticated subsystems for image acquisition, processing, database manipulation, graphics and statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes in one or more proteins among hundreds or thousands of unaltered proteins is the basic analytic problem. Keeping track of changes detected using these methods is also a major attribute of the system. A composite gel database may be "viewed" under different exploratory data analysis conditions and statistical differences and subtle patterns elucidated from "slices" of an effectively 3D database. Results can be presented in a variety of tables, plots or derived images and on workstations over wide area networks. Substantive GELLAB-II applications include: Ongoing studies of cadmium toxicity in urine (Myrick), Rett syndrome (Myrick), Vermont mercury study (Myrick), serum dioxin study (Myrick), fetal alcohol syndrome study (Myrick) - a new study will be neural tube defects (Myrick); 2D DNA gels for identifying differentially expressed genes (Rogan); investigation of radioactive accidents and fall-out from Chernobyl (Krekling). In a collaboration with Merril, we are developing a relational 2D gel interactive disease-protein-spot database system for answering queries relating proteins in plasma, serum, urine and CSF as a diagnostic tool. We continue to explore image conferencing of 2D gel images as well as other problem domains using Xconf. GELLAB-II has been exported to and is being supported at: CDC/Atlanta (Myrick), U. Norway (Krekling), Monsanto Research Labs (Leimgrubber et al.). This year, CDC has been the primary Beta test site for GELLAB-II. Additional changes to help continued collaboration with and exporting of enhanced graphical-interface versions of GELLAB has been actively pursued and is the primary activity this year.

分析方法正在继续发展和应用， GELLAB-II软件系统-一种用于 2D电泳凝胶图像组的分析。 而纳入 用于图像采集、处理、数据库 操作、图形和统计分析。它已被应用于一个 各种实验系统，其中定量和定性 一种或多种蛋白质在数百或数千种未改变的蛋白质中发生变化， 蛋白质是基本的分析问题。跟踪检测到的更改 使用这些方法也是该系统的一个主要特征。 复合 凝胶数据库可以在不同的探索性数据分析下“查看” 条件和统计差异和微妙的模式阐明， 有效的3D数据库的“切片”。 结果可以在 各种表格，绘图或衍生图像，并在工作站上广泛 区域网络。 GELLAB-II的实质性应用包括： 尿镉毒性研究（Myrick），Rett综合征（Myrick）， 佛蒙特州汞研究（Myrick），血清二恶英研究（Myrick），胎儿酒精 综合征研究（Myrick）-一项新的研究将神经管缺陷 （Myrick）;用于鉴定差异表达基因的2D DNA凝胶 （罗根）;放射性事故和沉降物的调查 切尔诺贝利（Krekling）。 在与Merril的合作中，我们正在开发一种关系型2D凝胶， 用于回答查询的交互式疾病蛋白点数据库系统 血浆、血清、尿液和CSF中的相关蛋白作为诊断工具。我们 继续探索2D凝胶图像以及其他 问题域使用Xconf。 GELLAB-II已出口到并得到以下机构的支持：CDC/Atlanta （Myrick），美国挪威（Krekling）、孟山都研究实验室（Leimgrubber et 等）。 今年，CDC一直是GELLAB-II的主要Beta测试网站。 其他更改有助于继续与 GELLAB的增强图形界面版本已经被积极地追求 是今年的主要活动。