COMPUTER AIDED TWO-DIMENSIONAL ELECTROPHORETIC GEL ANALYSIS

GELLAB是一个基于计算机的系统，用于分析一组 2D电泳胶。它包含复杂的子系统 用于图像采集和处理、数据库操作和 图表以及统计分析。它已被应用于一种 各种实验系统，其中定量和 数百种或多种蛋白质中的一种或多种发生质变 数以千计未改变的蛋白质是基本的分析问题。 使用统计方法跟踪检测到的变化 也是该系统的一个主要属性。它已被应用于 还帮助分析了一组成人白血病的凝胶 作为轴突再生过程中合成的轴突蛋白。这个 创建GELLAB的可导出版本的目标(该版本 将在相当强大的工作站微型计算机上运行- (实验室负担得起)正在积极推行。 过去一年的大部分时间都花在了升级软件上 和硬件从TOPS10环境迁移到Unix- 我们实验室从罗克维尔到弗雷德里克/FCRF的部分 在提出FCRF/LTB-ASCL上的各种元素时 网络。

GELLAB is a computer based system for the analysis of a set of 2D electrophretic gels. It incorporates sophisticated subsystems for image acquisition and processing, data base manipulation and graphics as well as statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes in one or more proteins among hundreds or thousands of unaltered proteins is the basic analytic problem. Keeping track of changes detected using the statistical methods is also a major attribute of the system. It has been applied to helping analyze a set of gels from adult human leukemias as well as axonal proteins synthesized during axonal regeneration. The objective of creating an exportable version of GELLAB (one that will run on reasonably powerful workstation microcomputers - affordable by a laboratory) is being actively pursued. Much of the time this past year was spent upgrading software and hardware from the TOPS10 environment to UNIX - moving our part of the laboratory from Rockville to Frederick/FCRF and in bringing up various elements on the FCRF/LTB-ASCL network.