COMPUTER AIDED TWO-DIMENSIONAL ELECTROPHORETIC GEL ANALYSIS

计算机辅助二维电泳凝胶分析

• 批准号: 3813367
• 负责人: P LEMKIN
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3813367
• 关键词: analytical chemistry; axon; blood /lymphatic neoplasm; cadmium; computer graphics /printing; cytotoxicity; gel electrophoresis; genetic manipulation; genetic transcription; image processing; molecular dynamics; molecular oncology; neoplasm /cancer diagnosis; nucleic acid sequence; prostate neoplasms; protein biosynthesis; protein sequence; statistics /biometry; tissue /cell culture

GELLAB is an exploratory data analysis system for the analysis of sets of 2D electrophoretic gel images. GELLAB incorporates sophisticated subsystems for image acquisition and processing, database manipulation and graphics as well as statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes in one or more proteins among hundreds or thousands of unaltered proteins is the basic analytic problem. Keeping track of changes detected using these methods is also a major attribute of the system. A composite gel database may be "viewed" under different exploratory data analysis conditions and statistical differences and subtle patterns elucidated from "slices" of an effectively 3D database. Results can be presented in a variety of tables, plots or derived images. The computer workstation based system, GELLAB-II, now runs under UNIX and uses X-windows for portable interactive graphics. Substantive applications include: analyzing sets of lymphocyte gels and long term cell lines from human hematological malignancies to identify potential marker proteins for possible microsequencing using a gas-phase sequenator (Lester); axonal proteins synthesized during axonal regeneration (Sonderegger); cadmium toxicity of epithelial cells (Sonderegger and Hunziker); cerebral endothelial cell morphology control (Bauer and Amberger); assess stability of long term cell lines (Alley).

GELLAB是一种探索性数据分析系统，用于分析 2D电泳凝胶图像。GELLAB集成了复杂的子系统 用于图像采集和处理、数据库操作和图形， 以及统计分析。它已被应用于各种 实验系统中，定量和定性的变化在一个 在成百上千的未改变的蛋白质中， 基本分析问题跟踪使用这些工具检测到的更改 方法也是系统的一个主要属性。复合凝胶数据库 可以在不同的探索性数据分析条件下“查看”， 统计差异和微妙的模式阐明了从“切片”的一个 3D数据库。结果可以在各种表格中呈现， 图或衍生图像。基于计算机工作站的系统，GELLAB-Ⅱ， 现在在UNIX下运行，并使用X-windows进行可移植的交互式图形。 实质性的应用包括：分析淋巴细胞凝胶组， 人类恶性血液病的长期细胞系， 用于可能的微测序的潜在标记蛋白 sequenator（Lester）;轴突再生过程中合成的轴突蛋白 （Sonderegger）;上皮细胞的镉毒性（Sonderegger和 Hunziker）;脑内皮细胞形态学对照（Bauer和 Amberger）;评估长期细胞系的稳定性（Alley）。