COMPUTER AIDED TWO-DIMENSIONAL ELECTROPHORETIC GEL ANALYSIS

继续开发便携式GELLAB-II系统。 GELLAB是 一个基于计算机的系统，用于分析2D 电泳凝胶。 GELLAB集成了复杂的子系统 用于图像采集和处理、数据库操作和 图表和统计分析。 它已被应用到 各种实验系统，其中定量和 一种或多种蛋白质在数百种或更多种蛋白质中发生质的变化， 成千上万的未改变的蛋白质是基本的分析问题。 跟踪使用这些统计方法检测到的变化 这也是该系统的一个重要特征。 相同的凝胶DB可以是 “看待”不同，并阐明其他统计差异。 结果可以以各种表格、图或推导的形式呈现。 图像. 实质性应用包括：分析凝胶组 从成年人白血病中识别潜在的标志蛋白 使用气相测序仪进行可能的微测序（Lester） 以及轴突再生过程中合成的轴突蛋白 （Sonderegger）. 创建可导出版本的目标是 GELLAB正在积极推进。 新系统GELLAB-II将 在UNIX下运行，并使用X-windows进行可移植的交互式图形。 稍后它将被导出到VMS和CRAY系统。 过去一年的大部分时间都花在了改造GELLAB-I上 软件从TOPS 10环境到GELLAB-II UNIX 环境 这包括新的UNIX软件， 与UNIX一起使用的SUN和microVAX硬件之间的差距 系统 从36位到32位硬件的主要转换工作 系统包括数据验证研究，以确保数据完整性 新的32位硬件和软件。

Continuing development of a portable GELLAB-II system. GELLAB is a computer based system for the analysis of sets of 2D electrophoretic gels. GELLAB incorporates sophisticated subsystems for image acquisition and processing, data base manipulation and graphics as well as statistical analysis. It has been applied to a variety of experimental systems in which quantitative and qualitative changes in one or more proteins among hundreds or thousands of unaltered proteins is the basic analytic problem. Keeping track of changes detected using these statistical methods is also a major attribute of the system. The same gel DB may be `viewed' differently and other statistical differences elucidated. Results can be presented in a variety of tables, plots or derived images. Substantive applications include: analyzing sets of gels from adult human leukemias to identify potential marker proteins for possible microsequencing using a gas-phase sequenator (Lester) as well as axonal proteins synthesized during axonal regeneration (Sonderegger). The objective of creating an exportable version of GELLAB is being actively pursued. The new system GELLAB-II, will run under UNIX and use X-windows for portable interactive graphics. Later it will be exported to VMS and CRAY systems. Much of the time this past year was spent converting GELLAB-I software from the TOPSlO environment to the GELLAB-II UNIX environment. This included new UNIX software necessary to fill in the gaps for the SUN and microVAX hardware used with the UNIX system. A major conversion effort from 36- to 32-bit hardware systems included data validation studies to ensure data integrity under the new 32-bit hardware and software.