LOCALIZATION AND CHARACTERIZATION OF BRAIN NEUROCHEMICALS

在过去的几年里，信息的爆炸已经成为一种 用分子生物学探针研究的结果 小鼠胚胎早期脑模式形成的遗传控制 发展。为了更全面地了解 神经系统，分子神经学家需要发现 在复杂的事件级联期间激活，最终导致 成熟神经系统的异种器官来自简单的上皮组织 出现在早期胚胎阶段的细胞。活跃的基因 在神经发育过程中(通过mRNA表达和 独特蛋白质的免疫染色)必须在 发育和成熟神经系统的结构异质性。 为了做到这一点，板凳分子科学家必须精通 对发育中的神经系统结构的识别。自.以来 很少有分子生物学家研究过这种细胞的解剖和形态。 成熟的神经系统，几乎没有人研究过大脑 形态发生，很可能大多数人都不能准确识别 发育中的大脑中的结构。显然，一个全面的 需要化学构造的小鼠脑图谱来提供这些 拥有他们所需要的信息的科学家们完全理解 他们的实验结果。 神经发育的许多分子研究都是在小鼠身上进行的， 尤其是基因敲除、突变和转基因小鼠。这就是为什么一本地图集 发展中的化学构造(归根结底，是异质性的一种衡量标准 对发育中的小鼠大脑进行基因表达)也是必要的。这个 如果地图集的基础是相同的 脑作为最常被研究的模型(小鼠)。 我们目前正在制作一份化学构造脑图谱，它显示了 老鼠的大脑。这将是一个彩色地图集，其中将包括大脑照片 和多巴胺、5-羟色胺、AChE的示意图(从E11到新生儿) (胆碱能)，Calretinin，Calbindin，含神经。我们的长期计划 意图是将这一知识应用于随后的实验，从而 我们将研究在治疗期间使用的各种药物的效果。 怀孕，可能会改变胎儿大脑的发育。地图集是 有望对那些积极从事学习的人产生深远的影响 发育中的胚胎大脑。

Over the last few years, an explosion of information has emerged as a result of the use of molecular biological probes to investigate the genetic control of pattern formation during early mouse embryonic brain development. In order to understand more fully the development of the nervous system, molecular neuroscientists need to discover genes that are active during the complex cascade of events that culminate in the heterogeneous organs of the mature nervous system from simple epithelial cells that appear at early embryonic stages. Genes that are active during neural development (as measured by mRNA expression and immunostaining of unique proteins) must be identified within the structural heterogeneity of the developing and maturing nervous system. In order to do this, the bench molecular scientist must be proficient in the identification of structures in the developing nervous system. Since few molecular biologists have studied the anatomy and morphology of the mature nervous system, and practically none have studied brain morphogenesis, it is likely that most are not able to accurately identify structures in the developing brain. Clearly, a comprehensive chemotectonic mouse brain atlas is needed that will provide these scientists with the information they require to fully understand the results of their experiments. Much molecular research in neural development is based in mouse, especially knockout, mutant and transgenic mice. This is why an atlas of the developing chemotectonics (ultimately, a measure of heterogeneous genetic expression) of the developing mouse brain is also needed. The identifications will simply be easier if the atlas is based in the same brain as the most often model (mouse) being studied. We are currently producing a chemotectonic brain atlas of the developing mouse brain. It will be a color atlas which will include brain photos and schematics (E11 to newborn) of dopamine, serotonin, AChE (cholinergic), calretinin, calbindin, containing nerves. Our long term intention is to apply this knowledge to subsequent experiments whereby we will study the effects of a variety of drugs that are used during pregnancy, that may alter fetal development of the brain. The atlas is expected to have a profound impact for those actively engaged in studying the developing embryonic brain.