AUTOIMMUNE ASPECTS OF DISEASE

僵人综合征是一种罕见的自身免疫性神经系统疾病 被认为是由于GABA能神经元的损伤所致。一位杰出的 针对GABA合成酶的体液免疫反应， 谷氨酸脱羧酶(GAD)，存在于大多数个体中 受到僵人综合症的影响。通过免疫组织化学方法检测 3例患者血清中GAD抗体的检测及临床意义 脑脊液1例。不同的稀释剂被放在 福尔马林切片固定了大鼠的大脑。一种荧光图案是 在大脑的许多区域中发现了与之相同的 用绵羊抗GAD血清进行观察。脑脊液 也表明GAD自身抗体的存在，尽管水平较低 效价。这些结果支持僵人综合症是一种 攻击中枢神经系统GABA能神经元的自身免疫性疾病。我们计划 未来的研究试图用僵人创造一个动物模型 血清。

Stiff-Man Syndrome is a rare autoimmune neurological disease that is thought to result from an impairment of GABAergic neurons. A prominent humoral immune response directed against the GABA-synthesizing enzyme, glutamic acid decarboxylase (GAD), occurs in the majority of individuals affected by Stiff-Man Syndrome. By immunohistochemistry we investigated the presence of GAD antibodies in the serum of 3 patients and the cerebrospinal fluid of 1 patient. A variety of dilutions were placed on sections of formalin fixed rat brains. A pattern of fluorescence was revealed in numerous regions of the brain that was identical to that observed when sheep antiserum to GAD was employed. The cerebrospinal fluid also indicated the presence of GAD autoantibodies, although at a lower titer. These results support the notion that the Stiff-Man Syndrome is an autoimmune disease that attacks GABAergic neurons of the CNS. We plan future studies to attempt to create an animal model using the Stiff-Man serum.