MOLECULAR, BIOLOGICAL, IMMUNOCHEMICAL CHYMOTRYPSIN STUDIES IN BRAIN & PERIPHERY

近年来，对这一问题的研究引起了人们的极大关注 蛋白酶，因为蛋白酶抑制剂已被证明是有效的 延长艾滋病患者的生命。 我们一直在研究 胰凝乳蛋白酶，一种蛋白酶，以前认为是 只在胰腺中合成 我们制造了一种兔抗血清 并发现胰凝乳蛋白酶存在于 胃和上皮细胞中绒毛的内分泌样细胞 肠的一部分 通过核糖核酸酶保护试验，我们还检测到 胰凝乳蛋白酶mRNA在大鼠胃、十二指肠、卵巢和脾脏中的表达。 我们 还在大鼠和人脑中鉴定了糜蛋白酶mRNA。 电泳和免疫印迹显示，胰凝乳蛋白酶带， 胃、十二指肠和大脑。 合成一种有效的蛋白酶， 胰凝乳蛋白酶在胰腺以外的组织中是显著的，这表明 在这些组织中的潜在生理和/或病理作用。

In recent times there has been a great focus of attention on the study of proteases, since protease inhibitors have been shown to be effective in extending the life of people with AIDS. We have been studying chymotrypsin, a protease enzyme that was previously believed to be synthesized only in the pancreas. We have generated a rabbit antiserum to human chymotrypsin and have found chymotrypsin to be present in endocrine-like cells of the villi in the stomach and in epithelial cells of the intestine. By a ribonuclease protection assay we also detected chymotrypsin mRNA in the rat stomach, duodenum, ovary and spleen. We have also identified chymotrypsin mRNA in the rat and human brain. Electrophoresis and immunoblotting revealed chymotrypsin bands in the stomach, duodenum and brain. Synthesis of a potent protease such as chymotrypsin in tissues other than pancreas is significant, suggesting a potential physiological and/or pathological role in these tissues.