LOCALIZATION AND CHARACTERIZATION OF BRAIN NEUROCHEMICALS

我们一直在准备一份显影的化学结构图谱 老鼠的大脑。我们已经揭示了E14大鼠前脑中的一个区域 含有大量CR免疫反应积聚的胚胎 (CR-IR)。我们认为这个区域就是鲜为人知的“丘脑隆起”。 (TE)。我们已经开发了一种方法来离散地剖析这个 地区建立富含钙调蛋白的细胞培养体系 神经元。这个系统约占神经元总数的40%。 这些细胞的数量。几种神经营养因子对血管紧张素转换酶的影响 钙调蛋白免疫反应(CR-IR)神经元的轴突生长 调查过了。脑源性神经营养因子(BDNF) 增加CR-IR细胞的数量。BDNF也增加了数量和 次级神经突起的长度。因此，BDNF似乎增加了 检测阈值以上的细胞中的CR浓度 免疫组织化学也刺激继发性发芽 CR-IR纤维。

We have been preparing a chemoarchitectonic atlas of the developing mouse brain. We have revealed a region in the forebrain of the E14 rat embryo which contains a large accumulation of CR-immunoreactivity (CR-IR). We believe this region is the little known "thalamic eminence" (TE). We have developed a method for the discrete dissection of this region in order to produce a cell culture system enriched in calretinin neurons. This system comprised approximately 40% of the total neuronal population of these cells. The effects of several neurotrophins on the neurite growth of calretinin-immunoreactive (CR-IR) neurons was investigated. Brain-derived neurotrophic factor (BDNF) significantly increased the number of CR-IR cells. BDNF also increased the number and length of secondary neurites. It therefore appears that BDNF increased the CR concentration in cells above the threshold of detection by immuno- histochemistry and also stimulates the sprouting of secondary CR-IR fibers.