LOCALIZATION AND CHARACTERIZATION OF BRAIN NEUROCHEMICALS

我们一直在准备一个化学结构图谱， 老鼠的大脑 我们揭示了E14大鼠前脑中的一个区域， 含有大量CR免疫反应性积聚的胚胎 （CR-IR）。 我们相信这个区域就是鲜为人知的“丘脑隆起” （TE）。 我们已经开发了一种方法，用于离散解剖这种 以产生富含钙视网膜蛋白的细胞培养系统 神经元 该系统包括约40%的总神经元 这些细胞的数量。 几种神经营养因子对神经元凋亡的影响 钙视黄蛋白免疫反应（CR-IR）神经元的轴突生长， 研究了脑源性神经营养因子（BDNF）显著 增加CR-IR细胞的数量。 BDNF也增加了数量， 次级神经突的长度。 因此，似乎BDNF增加了 细胞中的CR浓度高于检测阈值， 免疫组织化学，也刺激发芽的继发性 CR-IR纤维。

We have been preparing a chemoarchitectonic atlas of the developing mouse brain. We have revealed a region in the forebrain of the E14 rat embryo which contains a large accumulation of CR-immunoreactivity (CR-IR). We believe this region is the little known "thalamic eminence" (TE). We have developed a method for the discrete dissection of this region in order to produce a cell culture system enriched in calretinin neurons. This system comprised approximately 40% of the total neuronal population of these cells. The effects of several neurotrophins on the neurite growth of calretinin-immunoreactive (CR-IR) neurons was investigated. Brain-derived neurotrophic factor (BDNF) significantly increased the number of CR-IR cells. BDNF also increased the number and length of secondary neurites. It therefore appears that BDNF increased the CR concentration in cells above the threshold of detection by immuno- histochemistry and also stimulates the sprouting of secondary CR-IR fibers.