A STUDY OF PROTEINS WITHIN THE CNS BY TWO-DIMENSIONAL GEL ELECTROPHORESIS

我们一直在研究一种来自兔免疫前血清的新型自身抗体 免疫染色大脑的有髓神经。免疫前血清来自 一只兔子被发现含有选择性免疫染色的抗体 在老鼠的脑切片中发现了髓鞘。它还与少突胶质细胞反应， 在二维电泳凝胶的Western印迹上的培养中的细胞 该抗血清识别一个pI为碱性的46 kD蛋白。 在小鼠全脑匀浆的蛋白质印迹中， 强烈染色迁移到与2-相同位置的组分， 3-环核苷酸3-磷酸二酯酶（CNPL）带。虽然染色 CNP 1与自身抗体的反应比与孔 表征的抗CNP抗血清染色两种形式的CNP， CNP 2无染色。组织匀浆免疫染色和 具有自身抗体和已知抗CNP抗血清的髓磷脂样品 在髓鞘中显示了类似的CNP 1染色条带的富集。这 自身抗体可能有助于建立结构差异 CNP 1和CNP 2之间的差异，并证明 通过免疫组织化学定位两种形式。存在该 髓磷脂中的蛋白质可能有助于确定 髓鞘形成和自身免疫血清可能有助于结构研究 髓磷脂

We have been studying a novel autoantibody from a rabbit preimmune serum that immunostains myelinated nerves of the brain. Preimmune serum from a rabbit was found to contain antibodies that selectively immunostained myelin in brain sections of the rat. It also reacted with oligodendroglial cells in culture on Western blots of two-dimensional electrophoretic gels of rat cortex, this antiserum recognized a 46 kD protein with a basic pI. On Western blots of whole mouse brain homogenates, the autoantibody strongly stained a component that migrated to the same position as 2-, 3-cyclic nucleotide 3-phosphodiesterase (CNPL) band. Although the staining of the CNP1 was more intense with the autoantibody than that with a well characterized anti-CNP antiserum that stained both forms of CNP, there was no staining of CNP2. Comparison of the immunostaining of homogenate and myelin samples with the autoantibody and the known anti-CNP antiserum showed a similar enrichment of the stained CNP1 band in the myelin. This autoantibody may be useful in establishing the structural difference between CNP1 and CNP2, and for demonstrating possible differences in the localization of the two forms by immunohistochemistry. The presence of this protein within myelin may help identify possible cellular mechanisms of myelination, and the autoimmune serum might be useful in structural studies of myelin.