DETERMINATION OF GENETIC MUTATIONS IN ATYPICAL AND CANCEROUS BREAST LESIONS

非典型和癌性乳腺病变中基因突变的测定

• 批准号: 2464534
• 负责人: M MERINO
• 金额: --
• 依托单位: DIVISION OF CLINICAL SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2464534
• 关键词: breast neoplasms; clinical research; family genetics; female; gene mutation; genetic markers; human genetic material tag; human tissue; neoplasm /cancer genetics; polymerase chain reaction; preneoplastic state

It is still unclear the type of genetic alterations that patients with breast cancer have and (1) whether these alterations are different or similar to patients with familial history of breast cancer and (2) whether these changes occur in pre-malignant lesions, and whether any of these genetic changes have any prognostic implication. Utilizing paraffin-embedded material and following the technique described by Sukpanichnant, we will search by PCR amplification for a variety of genetic alterations. Specifically LOH at 17p, a known locus for p53 mutations will be evaluated in a group of patients with known aggressive disease and poor outcome.

目前还不清楚， 乳腺癌有（1）这些变化是否不同， 与有乳腺癌家族史的患者相似，以及（2）是否 这些变化发生在癌前病变中， 基因变化有任何预后意义。 使用石蜡包埋材料并遵循所述技术 通过Sukpanichnant，我们将通过PCR扩增寻找各种 基因改变特别是17 p的洛缺失，这是一个已知的p53基因位点 将在一组已知具有侵袭性的患者中评估突变。 疾病和不良后果。