TOOLS FOR ACQUISITION AND ANALYSIS OF MANY CELLULAR DATA

用于采集和分析多种细胞数据的工具

• 批准号: 2037905
• 负责人: KENNETH D MILLER
• 金额: $ 14.28万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-12-09 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2037905
• 关键词: action potentials; brain electrical activity; cell cell interaction; cell population study; computational neuroscience; computer data analysis; electrodes; model design /development; neocortex; statistics /biometry

There are a number of reasons why it is desirable to study the simultaneous responses of many cells at a single site in the mammalian neocortex. Such studies can determine the relative response fields and connectivity of nearby cells, yielding insight into local circuits and the origin of cortical response properties. Such studies can also test the idea that neural information may be encoded in synchronization or correlation of cell responses. Such insights into cortical function are fundamental to our understanding of normal mental function and its pathologies. The purpose of the present proposal is to develop accurate, automatic, quantifiable, objective, and real-time means of discriminating many cells in extracellular recording at a single site. This will be done by combining two methods, each of which has recently achieved significant progress on this discrimination problem: the tetrode method of recording, and Bayesian statistical methods of inferring the distinct waveforms underlying a recording. The tetrode method provides additional information, relative to single-electrode recording, which simplifies discrimination; while the Bayesian methods provide optimal and quantifiable discrimination based on the full information available in the recording. The combination of the two methods will produce a method that can discriminate 5-10 cells at a single site in a quantifiable, reproducible, and largely automatic manner, opening to a new quantity and quality of extracellular recording in the cerebral cortex.

有许多理由说明为什么需要研究 在哺乳动物中，许多细胞在一个位点同时响应 新皮层 这些研究可以确定相对响应场， 连接附近的细胞，从而深入了解局部电路， 皮层反应特性的起源 这样的研究也可以测试 神经信息可以同步编码的想法， 细胞反应的相关性。 这种对大脑皮层功能的深入了解 这是我们理解正常心理功能及其 病理学 本提案的目的是开发准确的，自动的， 可量化、客观和实时的方法来区分许多细胞 在细胞外记录在一个单一的网站。 会来做这项工作 结合两种方法，每一种方法最近都取得了重大进展， 在这一歧视问题上的进展： 记录和贝叶斯统计方法推断不同的 记录下的波形。 四极管方法提供了额外的 相对于单电极记录， 歧视;虽然贝叶斯方法提供了最佳和 根据现有的全部资料， 录音 这两种方法的结合将产生一种方法 它可以在一个可量化的， 可重复的，基本上是自动的方式，打开一个新的数量， 大脑皮层细胞外记录的质量。