DETECTING AND QUANTITATING UNCULTURABLE HUMAN PATHOGENS

检测和定量不可培养的人类病原体

• 批准号: 2716551
• 负责人: NORMAN G ANDERSON
• 金额: $ 37.5万
• 依托单位: LARGE SCALE BIOLOGY CORPORATION
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2716551
• 关键词: biotechnology; communicable disease diagnosis; density gradient ultracentrifugation; diagnosis design /evaluation; fluorescent dye /probe; lasers; method development; serology /serodiagnosis; virus characteristic; virus load

DESCRIPTION: (Adapted from the applicant's abstract) This proposal is to develop a rapid method for separating, identifying and counting small numbers of viral pathogens from tissues, blood, urine, water supplies, and natural waters, with emphasis on agents currently unculturable. It is based on the observation that in nature there are few particles of viral dimensions that have a narrow range of both sedimentation rates and isopycnic banding densities, which are not viruses. In Phase I, a prototype centrifugal concentration and microbanding system was constructed and tested which employs a density gradient, density standards, laser illumination and staining with DNA-binding fluorescent dyes. Virus particles from 4 mL samples have been concentrated to a cubic micron, yielding PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：(改编自申请人的摘要)本提案旨在 开发一种快速分离、识别和清点小颗粒的方法 来自组织、血液、尿液、供水和 天然水，重点是目前不可培养的菌剂。它是 根据观察，自然界中有很少的病毒颗粒 沉降率和沉降率的范围都很窄的维度 等位带密度，这不是病毒。在第一阶段， 设计了离心式浓缩微带系统样机。 构建和测试采用密度梯度、密度 标准、激光照射和DNA结合荧光染色 染料。4毫升样本中的病毒颗粒已浓缩到一立方体 美光，屈服 建议的商业应用：不可用